Pharmacokinetic and metabolic profiling studies of osmundacetone in rats by UPLC–MS/MS and UPLC–QE–Orbitrap–HRMS. (13th October 2021)

• Record Type: Journal Article
• Authors: Wu, Hao; Cao, Yijia; Wang, Jiaqi; Liu, Runhua; Sun, Yu; Zhang, Chenning; Sun, Yikun
• Is Part Of: Biomedical chromatography. Volume 36:Number 1(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-10-13
• Subjects: metabolism -- osmundacetone -- pharmacokinetics -- UPLC–MS/MS -- UPLC–QE–Orbitrap–HRMS; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.5251
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: Osmundacetone is a potential medicinal substance existing in ferns and has excellent antioxidant effects. This research aims to obtain the pharmacokinetic data for and metabolite products of osmundacetone. An UPLC–MS/MS quantitative method was established for the measurement of osmundacetonein in rat plasma over a linear range of 6.72–860.00 ng/ml. The signal to noise ratio of the lower limit of quantification was 60:1, the precision was <9.74% and the method had good selectivity and stability. The established method was successfully applied to the pharmacokinetic study of osmundacetone for the first time. Osmundacetone reached a peak at 0.25 h with a maximum value of 3283.33 μg/L. The apparent volume of distribution not multiplied by the bioavailability was 127.96 L/kg, and the half‐life of osmundacetone was 5.20 h. At the same time, an UPLC–QE–Orbitrap–HRMS method was established to identify metabolites in plasma, urine and feces for the first time. A total of 30 metabolites were identified and the metabolic profile of osmundacetone was defined. In general, we have established a mass spectrometry quantitative method for osmundacetone for the first time and characterized its metabolic characteristics in rats.