Evolution of aldehyde dehydrogenase genes and proteins in diploid and allotetraploid Xenopus frog species. (5th January 2022)

• Record Type: Journal Article
• Authors: Holmes, Roger S.
• Is Part Of: Chemico-biological interactions. Volume 351(2022)
• Publication Date: 2022-01-05
• Subjects: Aldehyde dehydrogenase -- ALDH -- Bioinformatics -- Enzyme structure -- Xenopus frogs -- Evolution -- Diploidy -- Tetraploidy; ALDH aldehyde dehydrogenase -- BLAST Basic Local Alignments Search Tool -- BLAT Blast-Like Alignment Tool -- NCBI National Center for Biotechnology Information; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2021.109671
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: At least 19 human aldehyde dehydrogenase (ALDH) genes and enzymes have been studied among vertebrate organisms. BLAT and BLAST analyses were undertaken of Xenopus tropicalis (western clawed frog) and Xenopus laevis (African clawed frog) genomes which are related diploid (N = 20) and allotetraploid (N = 36) species, respectively. The corresponding ALDH genes and proteins within these Xenopus genomes were identified and studied. Evidence is presented for tetraploid copies of 10 Xenopus laevis ALDH genes, whereas another 7 identified ALDH genes were diploid in nature. Xenopus laevis and Xenopus tropicalis ALDH amino acid sequences were highly homologous with the human enzymes, with the exception of the mitochondrial signal peptide sequences. Amino acids performing catalytic and structural roles were conserved and identified based on previous reports of 3D structures for the corresponding mammalian enzymes. Highlights: Bioinformatic studies were undertaken of clawed frog ALDH genes and proteins . Xenopus tropicalis ALDH genes were diploid in nature . Allotetraploid copies of 10 Xenopus laevis ALDH genes were observed . 7 diploid Xenopus laevis ALDH genes were also studied . Xenopus ALDH amino acid sequences were homologous with the human enzymes .