Polyamidoamine dendrimers of the third generation–chlorin e6 nanoconjugates: Nontoxic hybrid polymers with photodynamic activity. Issue 12 (28th October 2021)

• Record Type: Journal Article
• Authors: Díaz, Carola F.; Guzmán, Leonardo; Jiménez, Verónica A.; Alderete, Joel B.
• Is Part Of: Journal of applied polymer science. Volume 139:Issue 12(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-10-28
• Subjects: biomaterials -- biomedical applications -- dendrimers -- hyperbranched polymers and macrocycles; Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.51835
• Languages: English
• ISSNs: 0021-8995

Abstract

Abstract: Dendrimer‐based nanoconjugates play a key role as functional polymers for biomedical applications. In this work, the one‐pot conjugation of polyamidoamine dendrimers of the third generation (PAMAM‐G3) with the photosensitizer chlorin e6 (Ce6) is presented using 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide (EDC) and N‐hydroxysuccinimide (NHS) to mediate the synthesis, resulting in a 9% substitution degree according to nuclear magnetic resonance data. The PAMAM‐G3‐Ce6 nanoconjugate exhibits reduced cytotoxicity compared with the native dendrimer and efficient photodynamic activity against HeLa cancer cells after 1 h of incubation with a 100 μmol L −1 solution and 3 min of irradiation with red light‐emitting diode light (628 nm, 259 W m −2 ). Molecular dynamics simulations confirm that the PAMAM‐G3‐Ce6 system adopts a spherical structure and retains the internal dendritic cavities that enable drug encapsulation, which is relevant for the potential use of these hybrid polymers in combined chemo/photodynamic therapy. Abstract : Polyamidoamine dendrimers of the third generation–chlorin e6 nanoconjugates are easy to obtain and non‐toxic hybrid polymers with photodynamic activity and potential applications in combined chemo/photodynamic therapy.