Novel strain of Pseudoruminococcus massiliensis possesses traits important in gut adaptation and host-microbe interactions. (31st December 2022)

• Record Type: Journal Article
• Authors: Hiippala, Kaisa; Khan, Imran; Ronkainen, Aki; Boulund, Fredrik; Vähä-Mäkilä, Helena; Suutarinen, Maiju; Seifert, Maike; Engstrand, Lars; Satokari, Reetta
• Is Part Of: Gut microbes. Volume 14:Isuse 1(2022)
• Publication Date: 2022-12-31
• Subjects: Pseudoruminococcus -- pili -- adhesion -- gut microbiota -- next-generation probiotic -- starch degradation -- FMT; Gastrointestinal system -- Microbiology -- Periodicals; Microbiology -- Periodicals; Intestine, Small -- Periodicals; 616.3
• DOI: 10.1080/19490976.2021.2013761
• Languages: English
• ISSNs: 1949-0984

Abstract

ABSTRACT: Fecal microbiota transplantation (FMT) is an efficient treatment for recurrent Clostridioides difficile infection and currently investigated as a treatment for other intestinal and systemic diseases. Better understanding of the species potentially transferred in FMT is needed. We isolated from a healthy fecal donor a novel strain E10-96H of Pseudoruminococcus massiliensis, a recently described strictly anaerobic species currently represented only by the type strain. The whole genome sequence of E10-96H had over 98% similarity with the type strain. E10-96H carries 20 glycoside hydrolase encoding genes, degrades starch in vitro and thus may contribute to fiber degradation, cross-feeding of other species and butyrate production in the intestinal ecosystem. The strain carries pilus-like structures, harbors pilin genes in its genome and adheres to enterocytes in vitro but does not provoke a proinflammatory response. P. massiliensis seems to have commensal behavior with the host epithelium, and its role in intestinal ecology should be studied further.