Hybrid PCL/chitosan-PEO nanofibrous scaffolds incorporated with A. euchroma extract for skin tissue engineering application. (15th February 2022)

• Record Type: Journal Article
• Authors: Asghari, Fatemeh; Rabiei Faradonbeh, Davood; Malekshahi, Ziba Veisi; Nekounam, Houra; Ghaemi, Behnaz; Yousefpoor, Yaser; Ghanbari, Hossein; Faridi-Majidi, Reza
• Is Part Of: Carbohydrate polymers. Volume 278(2022)
• Publication Date: 2022-02-15
• Subjects: Biomaterials -- Skin tissue engineering -- Hybrid nanofibrous scaffolds -- Electrospinning; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118926
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Skin tissue engineering is an advanced method to repair and regenerate skin injuries. Recent research is focused on the development of scaffolds that are safe, bioactive, and cytocompatible. In this work, a new hybrid nanofibrous scaffold composed of polycaprolactone/chitosan-polyethylene oxide (PCL/Cs-PEO) incorporated with Arnebia euchroma ( A. euchroma) extract were synthesized by the two-nozzle electrospinning method. Then the synthesized scaffold was characterized for morphology, sustainability, chemical structure and properties. Moreover, to verify their potential in the burn wound healing process, biodegradation rate, contact angle, swelling properties, water vapor permeability, mechanical properties, antibacterial activity and drug release profile were measured. Furthermore, cytotoxicity and biocompatibility tests were performed on human dermal fibroblasts cell line via XTT and LDH assay. It is shown that the scaffold improved and increased proliferation during in-vitro studies. Thus, results confirm the efficacy and potential of the hybrid nanofibrous scaffold for skin tissue engineering. Graphical abstract: Unlabelled Image Highlights: Hybrid nanofibrous scaffolds containing A. euchroma will be suitable for wound treatment applications. The fabricated scaffolds demonstrated excellent viability and compatibility. This structure has good mechanical performance. The drug release profile of this structure showed sustain release which is suitable for wound dressing applications.