Aptamer‐functionalized Ti3C2‐MXene Nanosheets with One‐step Potentiometric Detection of Programmed Death‐ligand 1. Issue 1 (27th October 2021)

• Record Type: Journal Article
• Authors: Li, Bin; Pu, Wenyuan; Weng, Lingyan; Lyu, Peng; Xu, Houxi; Zhang, Wen; Ge, Lilin; Kwok, Hang Fai; Wu, Qinan
• Is Part Of: Electroanalysis. Volume 34:Issue 1(2022)
• Page Start: 2
• Page End: 7
• Publication Date: 2021-10-27
• Subjects: Potentiometric aptasensor -- Ti3C2-MXene nanosheets -- Programmed death-ligand 1 -- Electric potential -- Surface modified electrode; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.202100438
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: This communication describes a simple sensitive one‐step potentiometric aptasensing method for quantitative detection of a referenced therapeutic biomarker (programmed death‐ligand 1, PD−L1). The aptasensor is constructed by modifying PD−L1‐specific aptamer on Ti3 C2 ‐MXene nanosheets‐functionalized electrode. Introduction of PD−L1 induces the specific reaction between PD−L1 and aptamer, thereby resulting in the change of spatial structures. The surface electric potential of modified electrode is shifted upon addition of PD−L1 proteins before and after the reaction of aptamer with the analyte. Interestingly, potentiometric aptamer with Ti3 C2 ‐MXene nanosheets can achieve a higher sensitivity and a lower detection limit toward target PD−L1 relative to aptamer‐modified electrode. Experimental results indicated that the linear range and detection limit of using Ti3 C2 ‐MXene nanosheets were 0.01–100 ng mL −1 and 7.8 pg mL −1 PD−L1, respectively. Meanwhile, the specificity, reproducibility, storing stability and accuracy of potentiometric aptasensor are acceptable for the screening of PD−L1 in human serum samples. Abstract :