Glycine adversely affects enhanced biological phosphorus removal. (1st February 2022)

• Record Type: Journal Article
• Authors: Tian, Yucheng; Chen, Hang; Chen, Liping; Deng, Xuhan; Hu, Zekun; Wang, Cenchao; Wei, Chaohai; Qiu, Guanglei; Wuertz, Stefan
• Is Part Of: Water research. Volume 209(2022)
• Publication Date: 2022-02-01
• Subjects: Glycine -- Polyphosphate accumulating organisms (PAOs) -- Glycogen accumulating organisms (GAOs) -- Enhanced biological phosphorus removal (EBPR) -- P release -- P removal; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2021.117894
• Languages: English
• ISSNs: 0043-1354

Abstract

Highlights: Glycine induces phosphorus release without cellular uptake. Phosphorus release correlates with glycine concentration. Glycine hinders phosphorus uptake under aerobic conditions. Glycine may have induced an efflux mechanism of PAOs. The presence or introduction of glycine is detrimental to EBPR. Abstract: Enhanced biological phosphorus removal (EBPR) is used extensively in full-scale wastewater treatment plants for the removal of phosphorus. Despite previous evidence showing that glycine is a carbon source for a certain lineage of polyphosphate accumulating organisms (PAOs) such as Tetrasphaera, it is still unknown whether glycine can support EBPR. We observed an overall adverse effect of glycine on EBPR using activated sludge from both full-scale wastewater treatment plants and lab-scale reactors harboring distant and diverse PAOs and glycogen accumulating organisms (GAOs), including Candidatus Accumulibacter, Thiothrix, Tetrasphaera, Dechloromonas, Ca. Competibacter, and Defluviicoccus, among others. Glycine induced phosphorus (P) release under anaerobic conditions without being effectively taken up by cells. The induced P release rate correlated with glycine concentration in the range of 10 to 50 mg C/L. PAOs continued to release P in the presence of glycine under aerobic conditions without any evident P uptake. Under mixed carbon conditions, the occurrence of glycine did not seem to affect acetate uptake; however, it significantly reduced the rate of P uptake in the aerobic phase. Overall, glycine did not appear to be an effective carbon source for a majority of PAOs and GAOs in full-scale and lab-scale systems, and neither did other community members utilize glycine under anaerobic or aerobic conditions. Metatranscriptomic analysis showed the transcription of glycine cleavage T, P and H protein genes, but not of the L protein or the downstream genes in the glycine cleavage pathway, suggesting barriers to metabolizing glycine. The high transcription of a gene encoding a drug/metabolite transporter suggests a potential efflux mechanism, where glycine transported into the cells is in turn exported at the expense of ATP, resulting in P release without affecting the glycine concentration in solution. The ability of glycine to induce P release without cellular uptake suggests a way to effectively recover P from P-enriched waste sludge. Graphical abstract: Image, graphical abstract