Metabolic engineering for enhanced oil in biomass. (April 2019)

• Record Type: Journal Article
• Authors: Vanhercke, Thomas; Dyer, John M.; Mullen, Robert T.; Kilaru, Aruna; Rahman, Md. Mahbubur; Petrie, James R.; Green, Allan G.; Yurchenko, Olga; Singh, Surinder P.
• Is Part Of: Progress in lipid research. Volume 74(2019)
• Page Start: 103
• Page End: 129
• Publication Date: 2019-04
• Subjects: ABC ATP-binding cassette -- ABI abscisic acid insensitive -- ACCase acetyl-CoA carboxylase -- ACL ATP citrate lyase -- ACP acyl carrier protein -- AGPase ADP-glucose pyrophosphorylase -- ATGL adipose triglyceride lipase -- BADC biotin attachment domain-containing -- CFAs conjugated fatty acids -- CGI-58 comparative gene identification-58 -- CoA coenzyme A -- DAcT diacylglycerol acetyltransferase -- DAG diacylglycerol -- DCR defective in cuticular ridges -- DGAT acyl-CoA: diacylglycerol acyltransferase -- DHA docosahexaenoic -- DW dry weight -- EFAs epoxy fatty acids -- EPA eicosapentaenoic -- ER endoplasmic reticulum -- FAE1 fatty acid elongase 1 -- FAH12 fatty acid Δ12-hydroxylase -- FAS fatty acid synthase -- FIT2 fat storage-inducing transmembrane protein 2 -- FUS3 fusca3 -- G3P glycerol-3-phosphate -- GPAT G3P acyltransferase -- GRIK1 geminivirus rep-interacting kinase1 -- HFA hydroxy fatty acid -- JA jasmonic acid -- LACS long chain acyl-CoA synthetase -- IBA indole-3-butyric acid -- L1L LEC1-like -- LDAPs LD-associated proteins -- LDIP LDAP-interacting protein -- LDs lipid droplets -- LEC leafy cotyledon -- LPAAT lysophosphatidic acid acyltransferase -- MAG monoacylglycerol -- MCFAs medium-chain fatty acids -- MGAT acyl-CoA: monoacylglycerol acyltransferase -- MGD1 monogalactosyldiacylglycerol synthase 1 -- MGDG monogalactosyldiacylglycerol -- OLE1 oleosin1 -- OPDA 12-oxo-phytodienoic acid -- IAA indole-3-acetic acid -- PA phosphatidic acid -- PAP phosphatidic acid phosphatase -- PC phosphatidylcholine -- PDAT phospholipid:diacylglycerol acyltransferase -- PDHC pyruvate dehydrogenase complex -- PGM phosphoglucomutase -- RbohD respiratory burst oxidase homolog protein D -- RuBisCO ribulose-1, 5-bisphosphate carboxylase/oxygenase -- SDP1 sugar dependent 1 -- SRPPs small rubber particle proteins -- SSU small subunit -- T6P treh4alose-6-phosphate -- TAG triacylglycerol -- TGD trigalactosyldiacylglycerol -- VLC-PUFAs very-long-chain polyunsaturated fatty acids -- WRI1 wrinkled1; Metabolic engineering -- Biomass -- Oil -- Plant lipid metabolism; Lipids -- Periodicals; Lipids -- Periodicals; Lipides -- Périodiques; Lipiden; 572.57
• DOI: 10.1016/j.plipres.2019.02.002
• Languages: English
• ISSNs: 0163-7827

Abstract

Abstract: The world is hungry for energy. Plant oils in the form of triacylglycerol (TAG) are one of the most reduced storage forms of carbon found in nature and hence represent an excellent source of energy. The myriad of applications for plant oils range across foods, feeds, biofuels, and chemical feedstocks as a unique substitute for petroleum derivatives. Traditionally, plant oils are sourced either from oilseeds or tissues surrounding the seed (mesocarp). Most vegetative tissues, such as leaves and stems, however, accumulate relatively low levels of TAG. Since non-seed tissues constitute the majority of the plant biomass, metabolic engineering to improve their low-intrinsic TAG-biosynthetic capacity has recently attracted significant attention as a novel, sustainable and potentially high-yielding oil production platform. While initial attempts predominantly targeted single genes, recent combinatorial metabolic engineering strategies have focused on the simultaneous optimization of oil synthesis, packaging and degradation pathways (i.e., 'push, pull, package and protect'). This holistic approach has resulted in dramatic, seed-like TAG levels in vegetative tissues. With the first proof of concept hurdle addressed, new challenges and opportunities emerge, including engineering fatty acid profile, translation into agronomic crops, extraction, and downstream processing to deliver accessible and sustainable bioenergy.