Insight into multidrug-resistant microorganisms from microbial infected diabetic foot ulcers. Issue 2 (March 2019)

• Record Type: Journal Article
• Authors: Hassan, Mohamed A.; Tamer, Tamer M.; Rageh, Asmaa A.; Abou-Zeid, Alaa M.; Abd El-Zaher, Eman H.F.; Kenawy, El-Refaie
• Is Part Of: Diabetes & metabolic syndrome. Volume 13:Issue 2(2019)
• Page Start: 1261
• Page End: 1270
• Publication Date: 2019-03
• Subjects: Multidrug-resistant microorganisms -- Diabetic foot infections -- Methicillin-resistant Staphylococcus aureus (MRSA) -- Acinetobacter baumanni -- Candida albicans; Diabetes -- Periodicals; Metabolism -- Disorders -- Periodicals; Diabetes Mellitus -- Periodicals; Metabolic Diseases -- Periodicals; Diabète -- Périodiques; Métabolisme, Troubles du -- Périodiques; Endocrinologie -- Périodiques; Diabète -- Physiopathologie -- Périodiques; Diabetes; Metabolism -- Disorders; Electronic journals; Periodicals; 616.462
• DOI: 10.1016/j.dsx.2019.01.044
• Languages: English
• ISSNs: 1871-4021

Abstract

Abstract: Aim: This study was undertaken to inspect the preponderance of multidrug-resistant (MDR) microorganisms in microbial infected diabetic foot ulcers (DFUs) in north Egypt. Moreover, their later impact on the patients and previous antibiotic therapies were taken into consideration. Methods: To accomplish this goal, twenty-two of diabetic foot patients with purulent wounds were enrolled in this prospective study. These wounds were swabbed and the antibiotics susceptibility patterns of most virulent bacteria and yeast were studied. Furthermore, bacterial and yeast strains were identified using 16S rRNA and 16S rRNA nucleotide sequences, respectively, and following their phenotypic characteristics employing the VITEK 2 system. Results: Microbial profiles showed a predominance of monomicrobial infections (77.3%), while polymicrobial infections were found in 22.7%. A total of 24 bacterial isolates (15 Gram-positive and 9 Gram-negative) and four yeast isolates were perceived. Four bacteria were selected based on their resistance toward more than six of empirical antibiotics. They were identified and deposited in GenBank as Acinetobacter baumanni MT3 (KY421195), Staphylococcus aureus MT1 (KY421197), Klebsiella pneumonia MT2 (KY421196), and Staphylococcus aureus MT4 (KY421198). On the other hand, one strain belonged to yeast was opted and identified as Candida albicans MT5 (MG851796). Conclusion: These findings might effectively help to avert the severe complications of diabetic foot infections (DFIs) besides our endeavours to find new antimicrobial wound dressings. Highlights: Microbial profiles showed a predominance of monomicrobial infections of isolated organism from DFIs in Egypt. Imipenem demonstrated inhibition capacities toward the entire bacterial isolates except for A. baumanni MT3. Two methicillin-resistant S. aureus (MRSA) were detected as MDR to the most antibiotics. C. albicans MT5 was found as a co-infected organism with A. baumanni MT3. The lower limbs of diabetic foot patients infected by these organisms were amputated.