Mesenchymal stromal cell‐derived syndecan‐2 regulates the immune response during sepsis to foster bacterial clearance and resolution of inflammation. (15th August 2021)

• Record Type: Journal Article
• Authors: Han, Junwen; Shi, Yuanyuan; Willis, Gareth; Imani, Jewel; Kwon, Min‐Young; Li, Gu; Ayaub, Ehab; Ghanta, Sailaja; Ng, Julie; Hwang, Narae; Tsoyi, Konstantin; El‐Chemaly, Souheil; Kourembanas, Stella; Mitsialis, S. Alex; Rosas, Ivan O.; Liu, Xiaoli; Perrella, Mark A.
• Is Part Of: FEBS journal. Volume 289:Number 2(2022)
• Page Start: 417
• Page End: 435
• Publication Date: 2021-08-15
• Subjects: extracellular vesicles -- macrophage polarization -- mesenchymal stromal cells -- resolution of inflammation -- sepsis; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.16154
• Languages: English
• ISSNs: 1742-464X

Abstract

Abstract : Sepsis is a life‐threatening process related to a dysregulated host response to an underlying infection, which results in organ dysfunction and poor outcomes. Therapeutic strategies using mesenchymal stromal cells (MSCs) are under investigation for sepsis, with efforts to improve cellular utility. Syndecan (SDC) proteins are transmembrane proteoglycans involved with cellular signaling events including tissue repair and modulating inflammation. Bone marrow‐derived human MSCs express syndecan‐2 (SDC2) at a level higher than other SDC family members; thus, we explored SDC2 in MSC function. Administration of human MSCs silenced for SDC2 in experimental sepsis resulted in decreased bacterial clearance, and increased tissue injury and mortality compared with wild‐type MSCs. These findings were associated with a loss of resolution of inflammation in the peritoneal cavity, and higher levels of proinflammatory mediators in organs. MSCs silenced for SDC2 had a decreased ability to promote phagocytosis of apoptotic neutrophils by macrophages in the peritoneum, and also a diminished capability to convert macrophages from a proinflammatory to a proresolution phenotype via cellular or paracrine actions. Extracellular vesicles are a paracrine effector of MSCs that may contribute to resolution of inflammation, and their production was dramatically reduced in SDC2‐silenced human MSCs. Collectively, these data demonstrate the importance of SDC2 for cellular and paracrine function of human MSCs during sepsis. Abstract : Silencing of syndecan‐2 (shSDC2) in human mesenchymal stromal cells (hMSCs) leads to less effective bacterial clearance, more tissue injury due to persistent inflammation, and increased mortality in experimental sepsis. shSDC2 hMSCs have reduced efferocytosis and altered macrophage M2 polarization, which is associated with decreased production of extracellular vesicles.