Environment-sensitive fluorogens based on a GFP chromophore structural motif. (February 2022)

• Record Type: Journal Article
• Authors: Perfilov, Maxim M.; Zaitseva, Elvira R.; Smirnov, Alexander Yu; Mikhaylov, Andrey A.; Baleeva, Nadezhda S.; Myasnyanko, Ivan N.; Mishin, Alexander S.; Baranov, Mikhail S.
• Is Part Of: Dyes and pigments. Volume 198(2022)
• Publication Date: 2022-02
• Subjects: GFP -- Kaede -- Fluorogen -- Fluorescence -- Endoplasmic reticulum; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2021.110033
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: In this paper, we present a new series of arylidene-imidazolones with a structural motif of Green Fluorescent Protein chromophore with a pronounced solvent-dependent FQY variation. The identified structure-activity patterns made possible creation of a series of fluorogenic dyes with various positions of spectral maxima and Stokes shift. The developed dyes are cell-permeable and can be used for selective staining of the endoplasmic reticullum in living eukaryotic cells. Highlights: New series of arylidene-imidazolones with a pronounced solvent-dependent FQY variation are identified. Various derivatives characterized by pronounced bathochromic were created. Created compounds can be used for fluorescent staining of Endoplasmic Reticulum.