An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay. Issue 1 (2nd December 2021)

• Record Type: Journal Article
• Authors: Wang, Zhongxing; Zhao, Jing; Xu, Xinxin; Guo, Lingling; Xu, Liguang; Sun, Maozhong; Hu, Shudong; Kuang, Hua; Xu, Chuanlai; Li, Aike
• Is Part Of: Small methods. Volume 6:Issue 1(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-12-02
• Subjects: lateral flow assays -- materials -- optical properties -- quantitative detection -- strip reader; Nanotechnology -- Methodology -- Periodicals; Nanotechnology -- Periodicals; Periodicals; 620.5028
• DOI: 10.1002/smtd.202101143
• Languages: English
• ISSNs: 2366-9608

Abstract

Abstract: The development of the lateral flow assay (LFA) has received much attention in both academia and industry because of their broad applications to food safety, environmental monitoring, clinical diagnosis, and so forth. The user friendliness, low cost, and easy operation are the most attractive advantages of the LFA. In recent years, quantitative detection has become another focus of LFA development. Here, the most recent studies of quantitative LFAs are reviewed. First, the principles and corresponding formats of quantitative LFAs are introduced. In the biomaterial and nanomaterial sections, the detection, capture, and signal amplification biomolecules and the optical, fluorescent, luminescent, and magnetic labels used in LFAs are described. The invention of dedicated strip readers has drawn further interest in exploiting the better performance of LFAs. Therefore, next, the development of dedicated reader devices is described and the usefulness and specifications of these devices for LFAs are discussed. Finally, the applications of LFAs in the detection of metal ions, biotoxins, pathogenic microorganisms, veterinary drugs, and pesticides in the fields of food safety and environmental health and the detection of nucleic acids, biomarkers, and viruses in clinical analyses are summarized. Abstract : The lateral flow assay (LFA) has received much attention in both academia and industry because of their user friendliness, low cost, and easy operation. Here, the progress achieved of quantitative LFA during the last decade is summarized, and a helpful reference for solving the problems encountered in the development of quantitative LFA devices is provided.