Anti-diabetic activity of a sulfated galactoarabinan with unique structural characteristics from Cladophora oligoclada (Chlorophyta). (15th February 2022)

• Record Type: Journal Article
• Authors: He, Meijia; Hao, Jiejie; Feng, Changning; Yang, Yajing; Shao, Zhuling; Wang, Lei; Mao, Wenjun
• Is Part Of: Carbohydrate polymers. Volume 278(2022)
• Publication Date: 2022-02-15
• Subjects: Acetic acid (PubChem CID: 176) -- Adenosine triphosphate (PubChem CID:5957) -- l-Arabinose (PubChem CID: 439195) -- 2′, 7′-Dichlorofluorescin diacetate (PubChem CID: 77718) -- 3′-[4, 5-Dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (PubChem CID: 22288011) -- Ethanol (PubChem CID: 702) -- 1, 1, 1, 3, 3, 3-Hexafluoro-2-propanol (PubChem CID: 13529) -- Hexamethylene diamine (PubChem CID: 16402) -- Thioflavine T (PubChem CID: 16953) -- Uranyl acetate (PubChem CID: 10915); ATP adenosine triphosphate -- DCFH2-DA 2′, 7′-dichlorofluorescin diacetate -- FBS fetal bovine serum -- FITC fluorescin isothiocyanate -- HFIP 1, 1, 1, 3, 3, 3-hexafluoro-2-propanol -- hIAPP human islet amyloid polypeptide -- JC-1 J-Aggregate forming lipophilic cation 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethyl-benzimidazol-carbocyanine iodide -- MDA malonaldehyde -- MMP mitochondrial membrane potential -- NIT-1 mouse pancreatic β-cell line -- PBS phosphate buffer saline -- RFU relative fluorescence units -- ROS reactive oxygen species -- SOD superoxide dismutase -- ThT Thioflavin T -- T2DM type 2 diabetes mellitus; Cladophora oligoclada -- Sulfated polysaccharide -- Structural characterization -- hIAPP -- Anti-diabetic activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118933
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: The polysaccharide from green alga Cladophora oligoclada, OHSS2, was a sulfated galactoarabinan which was constituted by a backbone of (1 → 4)-β-l -arabinopyranose units with partial sulfate at C-3 of (1 → 4)-β-l -arabinopyranose units. The side chains containing (1 → 4)-β-l -arabinopyranose, (1 → 4)-β-d -galactopyranose and/or (1 → 4, 6)-β-d -galactopyranose units were in C-2/C-3 of (1 → 4)-β-l -arabinopyranose units. OHSS2 had strong anti-diabetic activity in vitro assessed by inhibition of human islet amyloid polypeptide (hIAPP) aggregation. The mechanism analysis of anti-diabetic activity showed that OHSS2 diminished the production of intracellular reactive oxygen species and alleviated hIAPP aggregation-induced oxidative stress in NIT-1 cells. OHSS2 stabilized mitochondrial membrane potential, and enhanced the mitochondrial complex I, II or III activity and ATP level. Thus, OHSS2 effectively protected mitochondria from hIAPP aggregation-induced damage. Furthermore, OHSS2 was co-localized with mitochondria and could have a direct influence on mitochondrial function. These results revealed that OHSS2 had potential as a novel anti-diabetic agent. Highlights: Polysaccharide from Cladophora oligoclada (Chlorophyta), OHSS2, is a sulfated galactoarabinan. OHSS2 is constituted by a backbone of (1 → 4)-β-L-arabinopyranosyl units partially sulfated at C-3. OHSS2 significantly inhibits hIAPP aggregation and has strong anti-diabetic activity in vitro . OHSS2 effectively protects mitochondria from hIAPP aggregation-induced damage. OHSS2 is co-localized with mitochondria and can have direct influence on mitochondrial function.