Comparative analysis of yak milk and bovine milk glycoprotein N/O-glycome by online HILIC-UV-ESI-MS/MS. (15th February 2022)

• Record Type: Journal Article
• Authors: Yang, Yuerong; Lu, Yu; Liu, Yinchuan; Pan, Yu; Ma, Hongjuan; Huang, Linjuan; Wang, Zhongfu
• Is Part Of: Carbohydrate polymers. Volume 278(2022)
• Publication Date: 2022-02-15
• Subjects: BM bovine milk -- GP Girard's reagent P -- HILIC-UV-ESI-MS/MS hydrophilic interaction liquid chromatography coupled with ultraviolet electrospray ionization-tandem mass spectrometry -- HPLC high performance liquid chromatography -- m/z mass-to-charge ratio -- Neu5Ac N-acetylneuraminic acid -- Neu5Gc N-glycolylneuraminic acid -- PMP 1-(d0/d5)phenyl-3-methyl-5-pyrazolone -- PNGase F peptide-N-glycosidase F -- YM yak milk; Bovine milk -- HILIC-MS/MS -- N/O-glycans -- Yak milk; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118918
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Yak milk (YM) has higher protein content than other bovine milk (BM) varieties. The bioactivity of milk glycoproteins is related to N / O -glycans. We qualitatively and quantitatively compared the N / O -glycome of YM and BM glycoproteins using stable isotope labeling combined with hydrophilic interaction chromatography and electrospray ionization mass spectrometry. We identified 79 and 78 N -glycans in YM and BM, respectively. Two N -glycans (H4N5F1A1; H5N4F1) were exclusive to YM. The content ratios of different types of N -glycans differed significantly between YM and BM, with sialylated N -glycans 2.33 times more abundant in YM. Five and seven O -glycans were detected in YM and BM, respectively. Two O -glycans (H1N2; H1N2A1) were exclusive to BM. The bi-sialylated O -glycan, H1N1A2, accounted for 56.1% of O -glycans in YM; it was 5.97 times more abundant in YM than in BM (equal volume basis). This study provides a theoretical basis for the future utilization of YM as a functional food. Graphical abstract: Unlabelled Image