Clonality of HIV-1– and HTLV-1–Infected Cells in Naturally Coinfected Individuals. (12th April 2021)

• Record Type: Journal Article
• Authors: Katsuya, Hiroo; Cook, Lucy B M; Rowan, Aileen G; Melamed, Anat; Turpin, Jocelyn; Ito, Jumpei; Islam, Saiful; Miyazato, Paola; Jek Yang Tan, Benjy; Matsuo, Misaki; Miyakawa, Toshikazu; Nakata, Hirotomo; Matsushita, Shuzo; Taylor, Graham P; Bangham, Charles R M; Kimura, Shinya; Satou, Yorifumi
• Is Part Of: Journal of infectious diseases. Volume 225:Number 2(2022)
• Page Start: 317
• Page End: 326
• Publication Date: 2021-04-12
• Subjects: HIV-1 -- HTLV-1 -- coinfection -- integration site analysis -- high-throughput sequencing; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiab202
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: Background: Coinfection with human immunodeficiency virus type 1 (HIV-1) and human T-cell leukemia virus type 1 (HTLV-1) diminishes the value of the CD4 + T-cell count in diagnosing AIDS, and increases the rate of HTLV-1–associated myelopathy. It remains elusive how HIV-1/HTLV-1 coinfection is related to such characteristics. We investigated the mutual effect of HIV-1/HTLV-1 coinfection on their integration sites (ISs) and clonal expansion. Methods: We extracted DNA from longitudinal peripheral blood samples from 7 HIV-1/HTLV-1 coinfected, and 12 HIV-1 and 13 HTLV-1 monoinfected individuals. Proviral loads (PVL) were quantified using real-time polymerase chain reaction (PCR). Viral ISs and clonality were quantified by ligation-mediated PCR followed by high-throughput sequencing. Results: PVL of both HIV-1 and HTLV-1 in coinfected individuals was significantly higher than that of the respective virus in monoinfected individuals. The degree of oligoclonality of both HIV-1– and HTLV-1–infected cells in coinfected individuals was also greater than in monoinfected subjects. ISs of HIV-1 in cases of coinfection were more frequently located in intergenic regions and transcriptionally silent regions, compared with HIV-1 monoinfected individuals. Conclusions: HIV-1/HTLV-1 coinfection makes an impact on the distribution of viral ISs and clonality of virus-infected cells and thus may alter the risks of both HTLV-1– and HIV-1–associated disease. Abstract : We investigated the mutual effect of HIV-1/HTLV-1 coinfection on their integration sites and the clonal expansion and found the degree of oligoclonality of both HIV-1– and HTLV-1–infected cells in coinfected individuals was greater than that in monoinfected subjects.