Genome-wide chromosomal association of Upf1 is linked to Pol II transcription in Schizosaccharomyces pombe. Issue 1 (20th December 2021)

• Record Type: Journal Article
• Authors: De, Sandip; Edwards, David M; Dwivedi, Vibha; Wang, Jianming; Varsally, Wazeer; Dixon, Hannah L; Singh, Anand K; Owuamalam, Precious O; Wright, Matthew T; Summers, Reece P; Hossain, Md Nazmul; Price, Emily M; Wojewodzic, Marcin W; Falciani, Francesco; Hodges, Nikolas J; Saponaro, Marco; Tanaka, Kayoko; Azzalin, Claus M; Baumann, Peter; Hebenstreit, Daniel; Brogna, Saverio
• Is Part Of: Nucleic acids research. Volume 50:Issue 1(2022)
• Page Start: 350
• Page End: 367
• Publication Date: 2021-12-20
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gkab1249
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: Although the RNA helicase Upf1 has hitherto been examined mostly in relation to its cytoplasmic role in nonsense mediated mRNA decay (NMD), here we report high-throughput ChIP data indicating genome-wide association of Upf1 with active genes in Schizosaccharomyces pombe . This association is RNase sensitive, correlates with Pol II transcription and mRNA expression levels. Changes in Pol II occupancy were detected in a Upf1 deficient ( upf1Δ ) strain, prevalently at genes showing a high Upf1 relative to Pol II association in wild-type. Additionally, an increased Ser2 Pol II signal was detected at all highly transcribed genes examined by ChIP-qPCR. Furthermore, upf1Δ cells are hypersensitive to the transcription elongation inhibitor 6-azauracil. A significant proportion of the genes associated with Upf1 in wild-type conditions are also mis-regulated in upf1Δ . These data envisage that by operating on the nascent transcript, Upf1 might influence Pol II phosphorylation and transcription.