Immunohistochemical analysis of vimentin expression in myocardial tissue from autopsy cases of ischemic heart disease. (February 2022)

• Record Type: Journal Article
• Authors: Kondo, Takeshi; Takahashi, Motonori; Yamasaki, Gentaro; Sugimoto, Marie; Kuse, Azumi; Morichika, Mai; Nakagawa, Kanako; Sakurada, Makoto; Asano, Migiwa; Ueno, Yasuhiro
• Is Part Of: Legal medicine. Volume 54(2022)
• Publication Date: 2022-02
• Subjects: Vimentin -- Immunohistochemistry -- Mesenchymal marker -- Myocardial remodeling -- Forensic pathology; Medical jurisprudence -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Medical jurisprudence; Periodicals; 614.1
• DOI: 10.1016/j.legalmed.2021.102003
• Languages: English
• ISSNs: 1344-6223

Abstract

Graphical abstract: Highlights: Vimentin is expressed in mesenchymal cells. Myocardium from ischemic heart disease was examined by vimentin immunohistochemistry. Vimentin immunostaining may be useful to supplement conventional staining techniques. Abstract: Vimentin is a type III intermediate filament cytoskeletal protein that is expressed mainly in cells of mesenchymal origin and is involved in a plethora of cellular functions. In this study, myocardial tissues from patients with ischemic heart disease and a mouse model of acute myocardial infarction were subjected to immunohistochemistry for vimentin. We first examined 26 neutral formalin-fixed, paraffin-embedded myocardial tissue samples from autopsies of patients that were diagnosed with ischemic heart disease within 48 h postmortem. Myocardial cells were negative for vimentin, whereas non-myocardial cells, including vascular endothelium, vascular smooth muscle, fibroblasts, nerve fibers, adipocytes and mesothelial cells, showed positivity. Elevated vimentin expression was observed around myocardial cells undergoing remodeling, suggesting fibroblastic and endothelial proliferation in these locations. By contrast, myocardial foci that were completely fibrotic did not show upregulated vimentin expression. Inflammatory foci including macrophages and neutrophils were clearly visualized with vimentin immunostaining. The same vimentin expression phenomena as those found in human samples were observed in the mouse model. Our study indicates that immunostaining of vimentin as a marker for myocardial remodeling and the dynamics of all non-myocardial cell types may be useful for supplementing conventional staining techniques currently used in the diagnosis of ischemic heart disease.