Changes to dentin extracellular matrix following treatment with plant-based polyphenols. (February 2022)

• Record Type: Journal Article
• Authors: Omar, Hussan; Gao, Feng; Yoo, Hyemin; Bim, Odair; Garcia, Carolina; LePard, Kathy J.; Mitchell, John C.; Agostini-Walesch, Gina; Carrilho, Marcela R.
• Is Part Of: Journal of the mechanical behavior of biomedical materials. Volume 126(2022)
• Publication Date: 2022-02
• Subjects: Dentin collagen -- Dentin ECM -- Plant extracts -- Polyphenols -- Biomechanics -- Biodegradation; Biomedical materials -- Periodicals; Biomedical materials -- Mechanical properties -- Periodicals; Biomedical materials; Biomedical materials -- Mechanical properties; Periodicals; Electronic journals; 610.28
• DOI: 10.1016/j.jmbbm.2021.105055
• Languages: English
• ISSNs: 1751-6161

Abstract

Abstract: This study investigated whether treatment with plant-based polyphenols (PB–P) affected the biochemical and/or biomechanical properties of dentin extracellular matrix (ECM). Three PB-Ps were evaluated: luteolin (LT), galangin (GL), and proanthocyanidin (PAC). Because dentin ECM requires demineralization before treatment, this study also assessed the effect of these PB-Ps on dentin demineralized by two different chemicals. Dentin samples from extracted third molars were obtained, sectioned, and randomly assigned for demineralization with either phosphoric acid (PA) or ethylenediaminetetraacetic acid (EDTA). Following demineralization, baseline infrared (IR) spectra and apparent elastic modulus (AE) of each specimen were independently acquired. Based upon these initial tests, samples were randomly assigned to one of the PB-P treatments to ensure that distribution of baseline AE was similar across treatment groups. IR and AE specimens were individually immersed in either 0.2% LT, 0.4% GL or 1% PAC for 2 min. IR spectra of treated samples were compared to baseline IR spectra, looking for any interaction of PB-Ps with the demineralized dentin. The IR spectrum and AE of each PB-P-treated specimen were compared with their own correspondent baseline measurement. The ability of PB-Ps to inhibit proteolytic activity of dentin ECM was assessed by the hydroxyproline assay. Finally, the effect of PB-Ps on immediate bond strength of a dental adhesive to PA- or EDTA-etched dentin was also evaluated. PB-Ps exhibited distinctively binding affinity to dentin ECM and promoted significant increase in AE. PB-P treatment reduced the degradation rate of dentin ECM without causing detrimental effect on immediate bond strength to dentin. Our work represents the first-time that LT and GL have been assessed as dentin ECM biomodifiers. Graphical abstract: Image 1 Highlights: Plant-based polyphenols had a favorable effect on dentin matrix degradation. Plant-based polyphenols enhanced the apparent modulus of elasticity of dentin matrix. Plant-based polyphenols did not interfere with the bond strength of dental adhesives to dentin. Binding affinity of polyphenols to dentin matrix depends on the demineralizing agent used.