First report on the isolation and genotyping of Toxoplasma gondii strains from free-range chickens in the state of Mato Grosso, Midwestern Brazil. (January 2022)

• Record Type: Journal Article
• Authors: Witter, Rute; Pena, Hilda Fátima Jesus; Maia, Maerle Oliveira; da Costa Freitas, Leodil; Almeida, Sayanne Luns Hatum; de Aguiar, Daniel Moura; Igarashi, Michelle; Alves, Bruna Farias; Gennari, Solange Maria; Pacheco, Richard Campos
• Is Part Of: Comparative immunology, microbiology and infectious diseases. Volume 80(2022)
• Publication Date: 2022-01
• Subjects: Bioassay -- Genetic diversity -- Toxoplasmosis -- Zoonosis; Communicable diseases in animals -- Periodicals; Veterinary immunology -- Periodicals; Veterinary microbiology -- Periodicals; Immunology -- Periodicals; Microbiology -- Periodicals; Communicable diseases -- Periodicals; Communicable Diseases -- immunology -- Periodicals; Communicable Diseases -- veterinary -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Veterinary Medicine -- Periodicals; Immunologie -- Périodiques; Microbiologie -- Périodiques; Maladies infectieuses -- Périodiques; Communicable diseases; Immunology; Microbiology; Electronic journals; Periodicals; 636.08969
• DOI: 10.1016/j.cimid.2021.101725
• Languages: English
• ISSNs: 0147-9571

Abstract

Abstract: In the present study, 51 strains of Toxoplasma gondii ( T. gondii ) were isolated from free-range chickens in the state of Mato Grosso, Midwestern Brazil, upon conducting bioassays in mice, and genotyped them using PCR-restriction fragment length polymorphism (PCR-RFLP) and 11 markers, including SAG1, SAG2 (5′3′SAG2 and alt. SAG2), SAG3, BTUB, GRA6, c22–8, c29–2, L358, PK1, Apico, and CS3. Fifty isolates were completely genotyped revealing 17 genotypes of T. gondii as follows: 12 matched using ToxoDB PCR-RFLP with the previously reported genotypes, including #6 type BrI ( n = 4), #8 type BrIII ( n = 7), #11 type BrII ( n = 3), #14 ( n = 1), #19 ( n = 1), #41 ( n = 1), #99 ( n = 1), #109 ( n = 4), #116 ( n = 1), #140 ( n = 2), #166 ( n = 9), #190 ( n = 1); and five genotypes have not been described before [#313 ( n = 6), #314 ( n = 1), #315 ( n = 1), #316 ( n = 1), #317 ( n = 1)]. Moreover, mixed infections were identified in five isolates (TgCkBrMT8, TgCkBrMT9, TgCkBrMT33, TgCkBrMT38, and TgCkBrMT41). Additionally, genotype #190 was reported for the first time in chickens from Brazil. Our results corroborate with previous studies on T. gondii isolates identified in chickens from Brazil, thereby confirming their diversity, a typicality, and possibility of co-infection due to different T. gondii strains present in the country. Highlights: Genetic diversity of Toxoplasma gondii strains from free-range chickens in Brazil in the Midwestern Brazil. Record of new genotypes of T. gondii from free-range chickens in Brazil using PCR-RFLP. Report of mixed infections in isolates obtained from free-range chickens. Genotype #190 reported in chickens from Brazil for the first time.