Yeast culture improved the growth performance, liver function, intestinal barrier and microbiota of juvenile largemouth bass (Micropterus salmoides) fed high-starch diet. Issue 120 (January 2022)

• Record Type: Journal Article
• Authors: Feng, Zhuandong; Zhong, Yunfei; He, Guanglun; Sun, Hao; Chen, Yongjun; Zhou, Wenhao; Lin, Shimei
• Is Part Of: Fish & shellfish immunology. Issue 120(2022)
• Page Start: 706
• Page End: 715
• Publication Date: 2022-01
• Subjects: High-starch diet -- Non-target metabonomics -- Antioxidant capacity -- Intestinal microbiota -- Micropterus salmoides; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2021.12.034
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The present study was conducted to investigate the effects of yeast culture on the growth, health and microflora of the juvenile largemouth bass fed high-starch diet. The experiment set three isonitrogenous and isolipidic diets, control (high-starch diet), HSY1 (high-starch diet with 1% yeast culture) and HSY3 (high-starch diet with 3% yeast culture). A feeding trial was conducted in largemouth bass juveniles for 8 weeks. The results indicated fish fed with 3% yeast culture not only could improve specific growth rate (SGR), but also significantly decreased hepatic lipid content, hepatic glycogen content, and hepatopancreas somatic index (HSI) compared with the control group ( p<0.05 ). The total superoxide dismutase (T-SOD) and catalase (CAT) activities of HSY3 group significantly increased while malondialdehyde (MDA) content significantly reduced in liver compared with the control group ( p<0.05 ). Meanwhile, the mRNA expression levels of hepatic Sod and Cat were up-regulated ( p<0.05 ), and liver metabolism showed 111 metabolites were significantly changed in HSY3 group, liver lipid metabolism pathway remarkably changed. Besides, the intestinal anti-inflammatory cytokines were significantly up-regulated, and the pro-inflammatory cytokines were significantly down-regulated as the inclusion of yeast culture ( p<0.05 ). Notably, HSY3 group diet up-regulated the expression of Zo-1, Claudin and Occludin in intestine compared with the other groups ( p<0.05 ). Serum d -lactate (D-lac), diamine oxidase (DAO) and lipopolysaccharide (LPS) decreased significantly with the inclusion of yeast culture ( p<0.05 ). Furthermore, the abundance of probiotics (such as Lactobacillus, Bacillus and Bifidobacterium ) increased significantly, and the abundance of intestinal potential pathogenic bacteria ( Plesiomonas ) decreased in HSY3 group ( p<0.05 ). The phenotypic analysis showed that gram-negative bacteria significantly decreased while gram-positive bacteria increased in HSY3 group ( p<0.05 ) . All in all, this study revealed that supplementation of 3% yeast culture can improve the growth performance and the health of juvenile largemouth bass, and has the potential to be used as an effective synbiotics for M. salmoides . Highlights: The 3% yeast culture could enhance growth performance and liver functiono of M. salmoides fed high-starch diet. The yeast culture could reinforce intestinal physical barrier and immune barrier of M. salmoides at high dietary starch levels. The yeast culture could regulate intestinal microbiota of M. salmoide .