Efficiency analysis of VersaPlex™ 27PY system in Central Indian Population: First report from Indian population. (February 2022)

• Record Type: Journal Article
• Authors: Shrivastava, Pankaj; Dixit, Shivani; Kumawat, R.K.; Srivastava, Ankit
• Is Part Of: Legal medicine. Volume 54(2022)
• Publication Date: 2022-02
• Subjects: Short tandem repeat (STR) -- Rapidly mutating Y-STRs -- Efficiency analysis -- VersaPlex™ -- Central Indians -- Forensic science; Medical jurisprudence -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Medical jurisprudence; Periodicals; 614.1
• DOI: 10.1016/j.legalmed.2021.101983
• Languages: English
• ISSNs: 1344-6223

Abstract

Highlights: Announcing first ever report based on the efficacy of VersaPlex™ 27PY system in Indian population. 217 Central Indians were voluntarily involved in this experiment. Sensitivity testing was performed to test the efficiency of this kit in forensic casework. In total 56 alleles were analysed with allele 11 at locus TPOX as observed to be the most frequent. Locus Penta E was found to be the most valuable in tested set of markers. Abstract: In the current scenario, DNA typing is the need of forensic science field due to its ability to provide results in much shorter time. In view of advancement of forensic DNA typing and incensement in the number of STRs markers, Promega offered a new VersaPlex™ 27PY system with 27 loci (23 autosomal STR loci, Amelogenin, DYS391 and two rapidly mutating Y-STR loci (DYS570 and DYS576)). In this study, the efficacy of "23 autosomal STR loci" for paternity testing and personal identification was demonstrated in Indian population. For this, 217 central Indians were tested and all the statistical parameters of forensic and population genetic interest were calculated. In addition, sensitivity of the kit was also tested for forensic casework. During investigation with VersaPlex™ 27PY system, allele 11 at locus TPOX was observed to be most frequent with the highest allelic frequency 0.432. Studied 23 loci showed valuable together with highest value of combined power of discrimination (CPD = 1), combined power of exclusion (CPI = 0.9999999989) and lowest value of combined matching probability (CPM = 7.92x10 -28 ).