Assessment of Triage DOA®, Status DS10, and DRIVEN-FLOW® M8-Z on-site drug screening kits for postmortem urine. (February 2022)

• Record Type: Journal Article
• Authors: Tanaka, Toshiko; Yoshida, Kosho; Kasai, Kentaro; Yoshizumi, Shiomi; Sato, Hiroaki
• Is Part Of: Legal medicine. Volume 54(2022)
• Publication Date: 2022-02
• Subjects: Mass analysis -- Autopsy -- Poisoning -- Benzodiazepine -- Amphetamines -- TCA -- Zolpidem; Medical jurisprudence -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Medical jurisprudence; Periodicals; 614.1
• DOI: 10.1016/j.legalmed.2021.101993
• Languages: English
• ISSNs: 1344-6223

Abstract

Highlights: Three types of drug screening kits were compared in 356 autopsy urine samples. Target drugs of Triage and AMP of Status showed false-positives due to putrefaction. The accuracy of Status and DF8 was comparable or better than Triage, except for TCA. Abstract: Based on the screening results of mass analyses using gas chromatography- mass spectrometry (GC–MS) and liquid chromatograph-tandem mass spectrometry (LC-MS/MS), we assessed the performance of Status DS10 (Status) and DRIVEN-FLOW® M8-Z (DF8), and compared the results with those of Triage DOA® (Triage) using 356 autopsy urine samples within one month of death. The sensitivity to benzodiazepines was 0.52 in Triage, 0.59 in Status, and 0.58 in DF8 with few false-positive cases. Triage detected triazolo-derivatives more easily than DF8. DF8 detected diazepam and nitro-benzodiazepines more easily than Status and Triage, with Status performing better than Triage. However, lorazepam detection with Status was difficult. There were 11 false-positive cases for amphetamines in Triage and 12 for Status-AMP at more than one week after death, but there were no false-positive in Status-MET and DF8. Tricyclic antidepressant (TCA) was detected in five cases by mass analysis, while there were 6 false-positive cases in Triage and 10 in both Status and DF8. In the TCA false-positive cases, tricyclic psychotics such as quetiapine, chlorpromazine, and carbamazepine existed. There were 23 true-positive and 6 false-positive cases for zolpidem in DF8 without false-negative cases. The accuracy of Status and DF8 for barbiturates or opiates was almost 1, but Triage was 0.98. There were no samples containing cocaine, THC, phencyclidine, or methadone. Based on the above, we conclude that Status and DF8 are comparable or slightly better than Triage, with fewer false-positive and fewer false-negative cases, except for TCA.