Structural characterization of three cytotoxic steroidal saponins from the leaves of Agave desmetiana hort. (March 2022)

• Record Type: Journal Article
• Authors: Gabr, Nagwan M.; Ghaly, Neveen S.; Mina, Suzan A.
• Is Part Of: Phytochemistry. Volume 195(2022)
• Publication Date: 2022-03
• Subjects: Agave desmetiana hort. -- Agavaceae -- Cytotoxicity -- Steroidal saponins -- Tigogenin; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2021.113057
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: Three steroidal saponins detected by LC-MS were isolated from the leaves of Agave desmetiana hort. The three saponins were characterized as; Tigogenin 3 – [{O – β – D - xylopyranosyl (1 → 2) – α – L–rhamnopyranosyl (1 → 3)}–β–D– glucopyranoside), Tigogenin– 3 - ([O- α –L–rhamnopyranosyl (1 → 3) – β – D - glalactopyranosyl (1 → 2)] – β – D – glucopyranoside) and Tigogenin– 3 - ([{O – α – L – rhamnopyranosyl (1 → 4)} – β – D - galactopyranosyl (1 → 3) – β – D – xylopyranosyl (1 → 2)] – β – D – glucopyranoside). Identification and structure elucidation of the isolates were done via 1D and 2D NMR techniques, and chemical methods. Cytotoxic activity for the crude saponins and the three isolated compounds were evaluated against Hepg-2 and Mcf-7 cell lines. Compound 2 proved to be the most cytotoxic against tested cell lines with an IC50 2.97 and 2.49 μg/ml respectively. Graphical abstract: Image 1 Highlights: Three undescribed steroidal saponins were isolated from Agave desmetiana hort. Their structures were determined by 1D, 2D NMR and chemical methods. Compounds isolated showed promising results against Hepg-2 and Mcf-7 cell lines.