Performance and microbial features of Anammox in a single-phase reactor under progressive nitrogen loading rates for wastewater treatment plants. Issue 1 (February 2022)

• Record Type: Journal Article
• Authors: Bonassa, Gabriela; Venturin, Bruno; Bolsan, Alice Chiapetti; Hollas, Camila Ester; Candido, Daniela; Rodrigues, Heloisa Campeão; Cantão, Mauricio Egidio; Ibelli, Adriana Mercia Guaratini; De Prá, Marina Celant; Antes, Fabiane Goldschmidt; Kunz, Airton
• Is Part Of: Journal of environmental chemical engineering. Volume 10:Issue 1(2022)
• Publication Date: 2022-02
• Subjects: Anammox -- Nitrogen removal -- Nitrogen performance -- Microbial community; Chemical engineering -- Environmental aspects -- Periodicals; Environmental engineering -- Periodicals; Chemical engineering -- Environmental aspects; Environmental engineering; Periodicals; 660.0286
• DOI: 10.1016/j.jece.2021.107028
• Languages: English
• ISSNs: 2213-2929

Abstract

Abstract: The performance of a new one-phase reactor, called NITRAMMOX®, fed with increasing nitrogen loading rates (NLR) from swine wastewater was evaluated. The aim of the present study was to investigate the stability of Partial Nitritation/Anammox (PN/A) process in a new single-phase reactor under 500 mg NH3 -N L −1 (2.04 ± 0.30 g N L −1 d −1 ). NITRAMMOX® reactor was operated under progressive nitrogen loading rates, and sludge samples were collected and analyzed for microorganism community, extracellular polymeric substance (EPS) and protein/polysaccharides ratio (PN/PS) evaluation at each experimental phase. The NITRAMMOX® reactor can be operated until a nitrogen loading rate of 2 g N L −1 d −1, with nitrogen removal efficiency higher than 80%. On these conditions, no inhibitions by substrate or nitrite were achieved, and the specific ammonia consumption rate ( q max ) did not decrease (35.49 mg NH3 -N gVSS −1 h −1 ). The main bacteria at the phylum and genus level had shifted according to high-throughput Miseq sequencing analyses. Candidatus Brocadia became the dominant Anammox genus under high-nitrogen loading rate (32.55%), which correlated positively with TN removal efficiency, EPS, protein/polysaccharide (PN/PS) and specific bacteria activity, respectively. NITRAMMOX® reactor showed good performance and robustness treating wastewater with low COD/N, with stability to substrate loading shocks. Graphical Abstract: ga1 Highlights: NITRAMMOX® successfully treats wastewater with up to 500 mgNH3 -N L −1 . With a NLR of 2 g N L −1 d −1 and ~60 mgTOC L −1 no inhibition was achieved. Candidatus Brocadia was the dominant genus to high NLR. Higher EPS was achieved with N-removal stability and higher bacteria activity.