Global meta-analysis of terrestrial nitrous oxide emissions and associated functional genes under nitrogen addition. (February 2022)

• Record Type: Journal Article
• Authors: You, Luncheng; Ros, Gerard H.; Chen, Yongliang; Yang, Xue; Cui, Zhenling; Liu, Xuejun; Jiang, Rongfeng; Zhang, Fusuo; de Vries, Wim
• Is Part Of: Soil biology and biochemistry. Volume 165(2022)
• Publication Date: 2022-02
• Subjects: Abundance -- Diversity -- Functional gene -- Meta-analysis -- Nitrogen addition -- Nitrous oxide; Soil biochemistry -- Periodicals; Soil biology -- Periodicals; Sols -- Biochimie -- Périodiques; Sols -- Biologie -- Périodiques; Sols -- Microbiologie -- Périodiques; Bodembiologie; Biochemie; 631.46
• DOI: 10.1016/j.soilbio.2021.108523
• Languages: English
• ISSNs: 0038-0717

Abstract

Abstract: Functional genes involved in nitrogen (N) cycling regulate soil nitrification, denitrification and N2 O emissions. However, the general patterns and variability of N functional genes in response to N addition, and their association with N2 O emission have not been synthesized for terrestrial ecosystems. We synthesized 2068 observations from 144 papers to explore the impact of N addition on the abundance and diversity of N functional genes, and their relationship to N2 O emissions in croplands, grasslands and forests on a global scale. In croplands, N addition increased N2 O emissions (109%), the abundance of ammonia-oxidizing archaea (AOA) (19%), ammonia-oxidizing bacteria (AOB) (95%), nirK (52%), nirS (40%) and nosZ (41%), and the diversity of AOB (15%), nirS (12%) and nosZ (11%). In grassland, N addition increased AOB abundance (130%) and decreased the abundance of nirS (−99%) and nosZ (−58%) genes, but in forests, significant effects were only found for the abundance of AOA (35%) and AOB (121%). N2 O emission was negatively correlated with the abundance of nosZ, but positively correlated with the abundance of AOA and AOB. Apart from the abundance of functional AOA, AOB and nosZ genes, climate variables (precipitation and temperature), and available N concentrations were the main factors explaining the variation in N2 O emission with N addition, as shown by random forest analysis. These findings indicate that impacts on N functional genes that encode enzymes involved in nitrification (AOA, AOB) and in the transformation of N2 O to N2 ( nos Z) are the main mechanisms behind the effect on N fertilizer-induced N2 O emissions. Highlights: Nitrogen addition increased the abundance of AOA, AOB, nirK, nirS and nosZ . N2 O emission was negatively correlated with the abundance of nosZ gene. N2 O emission was positively correlated with the AOA and AOB abundance. AOA, AOB and nosZ abundance were main factors affecting N2 O emission. Climate variables and available N concentrations were additional main factors.