Dapsone reduced acetic acid-induced inflammatory response in rat colon tissue through inhibition of NF-kB signaling pathway. (2nd November 2019)

• Record Type: Journal Article
• Authors: Rashidian, Amir; Rashki, Asma; Abdollahi, Alireza; Haddadi, Nazgol-Sadat; Chamanara, Mohsen; Mumtaz, Faiza; Dehpour, Ahmad Reza
• Is Part Of: Immunopharmacology and immunotoxicology. Volume 41:Number 6(2019)
• Page Start: 607
• Page End: 613
• Publication Date: 2019-11-02
• Subjects: Dapsone -- ulcerative colitis -- acetic acid -- NF-kB -- rats; Immunopharmacology -- Periodicals; Immunotoxicology -- Periodicals; Antibody-toxin conjugates -- Periodicals; Immunology -- Periodicals; 615.37
• DOI: 10.1080/08923973.2019.1678635
• Languages: English
• ISSNs: 0892-3973

Abstract

Abstract: Aim: The purpose of this study is to examine the protective effects of Dapsone on inflammation of intestinal tissue through inhibition of NF-kB pathway in acetic acid-induced colitis in rats. Methods: Acute colitis was produced by intra-rectal instillation of 2 mL of 4% acetic acid diluted in normal saline. Then, two hours after induction of colitis, DMSO as vehicle, dexamethasone (2 mg/kg) and dapsone (12.5 mg/kg) were given to the animals intraperitoneally (i.p.) and continued for five following days. Evaluation of macroscopic and microscopic damages were done. Myeloid peroxidase enzyme (MPO) activity was measured by a biochemical technique. Moreover, tumor necrosis factor-α (TNF-α) activity was identified by ELISA, and the expression level of pNF-kB protein was evaluated by immunohistochemistry (IHC). Results: Dexamethasone (2 mg/kg) and dapsone (12.5 mg/kg) decreased the macroscopic and microscopic damages compared with acetic acid group ( p ˂ .001). Additionally, these agents decreased the activity of MPO ( p ˂ .001), TNF-α ( p ˂ .001) and the expression level of p-NF-kB ( p ˂ .001) in rat colon tissue compared with the acetic acid group. Conclusion: It is proposed that the anti-inflammatory activity of dapsone on acetic acid-induced colitis in rats may involve the inhibition of NF-kB pathway.