A label‐free and signal‐amplifiable assay method for colorimetric detection of carcinoembryonic antigen. Issue 2 (8th December 2021)

• Record Type: Journal Article
• Authors: Li, Peng; Li, Wenqin; Xie, Zhuohao; Zhan, Haonan; Deng, Liehua; Huang, Jiahao
• Is Part Of: Biotechnology and bioengineering. Volume 119:Issue 2(2022)
• Page Start: 504
• Page End: 512
• Publication Date: 2021-12-08
• Subjects: Label‐ and enzyme‐free -- aptamer -- signal amplification -- hemin/G‐quadruplex -- hybridization chain reaction; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.28003
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: In this work, an innovative colorimetric assay method for the determination of carcinoembryonic antigen is developed with aptamer probes utilized as recognition element. DNA hybridization chain reaction is used as signal amplification technique, and peroxidase‐mimicking hemin/G‐quadruplex‐assisted catalytic oxidation of 2, 2'‐azino‐bis‐3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) is deployed as signal reporting mechanism. The detection principle was firstly verified by using gel electrophoresis analysis and absorbance measurements. After condition optimization, a detection limit was theoretically determined as 24.8 ng/ml. Furthermore, the method exhibited good selectivity and satisfactory recovery rates (92.2%–108.6%) in serum samples. Moreover, the sensing scheme is easily extended for the detection of other analytes via similar target‐aptamer recognition principle. To sum up, this is an enzyme‐ and label‐free, cost‐effective yet signal‐amplifiable assay scheme for the determination of tumor markers with promising simplicity and selectivity, practical utility, and potential universality. Abstract : An antibody‐ and label‐free assay method is developed by Huang and coworkers for isothermal and colorimetric determination of carcinoembryonic antigen which possesses advantages including simplicity and selectivity, applicability in real sample, and potential universality. The proposed strategy holds a promising potential to become a reliable and technology in biomedical applications.