Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG. (21st October 2021)

• Record Type: Journal Article
• Authors: Arimura, Yuki; Minato, Yusuke; Wada, Takayuki; Nakayama, Masaaki; Ryumon, Ayako; Hirata, Nao; Nakajima, Chie; Suzuki, Yasuhiko; Ato, Manabu; Kobayashi, Kazuo; Ohara, Naoko; Iida, Seiji; Ohara, Naoya
• Is Part Of: Microbiology and immunology. Volume 66:Number 1(2022)
• Page Start: 10
• Page End: 14
• Publication Date: 2021-10-21
• Subjects: BCG -- Mycobacterium -- thymidylate synthase -- ThyX; Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12944
• Languages: English
• ISSNs: 0385-5600

Abstract

Abstract: Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin‐dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non‐essentiality of thyX under a certain genetic background.