Progress in methodologies and quality‐control strategies in protein cross‐linking mass spectrometry. Issue 23 (28th October 2021)

• Record Type: Journal Article
• Authors: Yugandhar, Kumar; Zhao, Qiuye; Gupta, Shobhita; Xiong, Dapeng; Yu, Haiyuan
• Is Part Of: Proteomics. Volume 21:Issue 23/24(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-10-28
• Subjects: cross‐linking -- interaction network -- mass spectrometry -- protein‐protein -- proteomics; Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.202100145
• Languages: English
• ISSNs: 1615-9853

Abstract

Abstract: Deciphering the interaction networks and structural dynamics of proteins is pivotal to better understanding their biological functions. Cross‐linking mass spectrometry (XL‐MS) is a powerful and increasingly popular technology that provides information about protein‐protein interactions and their structural constraints for individual proteins and multiprotein complexes on a proteome‐scale. In this review, we first assess the coverage and depth of the XL‐MS technique by utilizing publicly available datasets. We then delve into the progress in XL‐MS experimental and computational methodologies and examine different quality‐control strategies reported in the literature. Finally, we discuss the progress in XL‐MS applications along with the scope for future improvements.