Increased zinc finger protein zFOC1 transcripts in gastric cancer compared with normal gastric tissue. Issue 3 (1st June 2003)

• Record Type: Journal Article
• Authors: Stephen, R L; Crabtree, J E; Yoshimura, T; Clayton, C L; Dixon, M F; Robinson, P A
• Is Part Of: Journal of clinical pathology. Volume 56:Issue 3(2003)
• Page Start: 167
• Page End: 171
• Publication Date: 2003-06-01
• Subjects: gastric cancer -- zFOC1 -- zinc finger protein -- cDNA array -- Helicobacter pylori; EST, expressed sequence tag -- GAPDH, glyceraldehyde 3 phosphate dehydrogenase -- PAI, pathogenicity island -- RT-PCR, reverse transcription polymerase chain reaction -- SDS, sodium dodecyl sulfate -- SCC, saline sodium citrate -- UTR, untranslated region; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/mp.56.3.167
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Background: Screening of cDNA arrays of the IMAGE library identified human zFOC1 as a differentially expressed cDNA that was upregulated in KATO III gastric cancer cells following stimulation with the gastric pathogen Helicobacter pylori. Aims: To determine the expression of zFOC1 in gastric mucosa with and without H pylori infection and in patients with gastric cancer. Results: zFOC1 is localised on chromosome 12q24.3 and encodes a zinc finger protein. Expression studies in human H pylori infected and uninfected gastric biopsies, gastric tumours, and gastric cancer cell lines revealed that zFOCI gene transcripts are significantly higher in gastric cancer than in non-cancerous gastric tissues. Conclusions: The zFOC1 gene appears to be a tumour marker associated with gastric cancer.