A sensitive fluorescent assay for measuring the cysteine protease activity of Der p 1, a major allergen from the dust mite Dermatophagoides pteronyssinus. Issue 4 (August 1998)

• Record Type: Journal Article
• Authors: Schulz, O; Sewell, H F; Shakib, F
• Is Part Of: Journal of clinical pathology. Volume 51:Issue 4(1998)
• Page Start: 222
• Page End: 224
• Publication Date: 1998-08
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/mp.51.4.222
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : The potent allergenicity of Der p 1, a major allergen of the house dust mite Dermatophagoides pteronyssinus, is thought to be related to its cysteine protease activity. Therefore, there is considerable interest in developing a sensitive assay for measuring Der p 1 activity to screen for specific inhibitors. This study demonstrates for the first time that the activity of Der p 1 can be measured conveniently in a continuous rate assay with the fluorogenic substrate Boc-Gln-Ala-Arg-AMC (K(m) = 280 microM and kcat/K(m) = 4.6 x 10(3)/M/s).