High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining. Issue 5 (October 1997)

• Record Type: Journal Article
• Authors: Xie, T; Ho, S L; Ma, O C
• Is Part Of: Journal of clinical pathology. Volume 50:Issue 5(1997)
• Page Start: 276
• Page End: 278
• Publication Date: 1997-10
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/mp.50.5.276
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Single strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments.