Isolation of DNA from archival Papanicolaou stained cytological smears using a simple salting-out procedure. Issue 1 (February 1995)

• Record Type: Journal Article
• Authors: Poljak, M; Barlič, J; Seme, K; Avšič-Županc, T; Zore, A
• Is Part Of: Journal of clinical pathology. Volume 48:Issue 1(1995)
• Page Start: M55
• Page End: M56
• Publication Date: 1995-02
• Subjects: Polymerase chain reaction; DNA isolation; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/mp.48.1.M55
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : DNA from archival Papanicolaou stained and unstained cytological smears was successfully isolated using a simple, rapid and inexpensive salting-out procedure. The quality of DNA was controlled by polymerase chain reaction (PCR) amplification of segments of the human β-globin, human β-actin and human papillomavirus L1 genes. Only negligible differences in amplification efficiency were observed between DNA isolated from stained and unstained smears. The salting-out procedure is a more rapid method for the isolation of DNA than phenol-chloroform extraction and may be used in instances where fresh or cryopreserved clinical specimens are not available.