A rapid and efficient method for DNA extraction from paraffin wax embedded tissue for PCR amplification. Issue 3 (June 1996)

• Record Type: Journal Article
• Authors: Morgan, Kevin; Lam, Letty; Kalsheker, Noor
• Is Part Of: Journal of clinical pathology. Volume 49:Issue 3(1996)
• Page Start: M179
• Page End: M180
• Publication Date: 1996-06
• Subjects: PCR; DNA extraction; paraffin wax sections; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/mp.49.3.M179
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : DNA from archival, formaldehyde fixed, paraffin wax embedded human tissue, suitable for amplification by the polymerase chain reaction (PCR), was obtained using a microwave method based on the capture of DNA by magnetic beads. Fragments of the α-1-antitrypsin gene (AAT) and the apolipoprotein E gene (APOE) were amplified successfully from human liver and brain tissue, respectively. This procedure provides a more rapid, simple and efficient method for reproducibly obtaining DNA from preserved tissue that has been kept in storage for up to 30 years.