Effect of Wnt-1 antisense RNA on the outgrowth of a mammary adenocarcinoma cell line expressing that oncogene. Issue 3 (June 1996)

• Record Type: Journal Article
• Authors: Polanec, J; Pavelic, Z P; Myers, W L
• Is Part Of: Journal of clinical pathology. Volume 49:Issue 3(1996)
• Page Start: M166
• Page End: M169
• Publication Date: 1996-06
• Subjects: mammary adenocarcinoma; cell lines; Wnt-1 oncogene; RNA; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/mp.49.3.M166
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Aims —To investigate the effect of Wnt-1 antisense RNA on the outgrowth of a mammary tumour cell line expressing that oncogene. Methods —A plasmid (pMT 70), containing Wnt-1 cDNA, was cut with appropriate enzymes and inserted into a eukaryotic expression vector (pMAMneo). A mammary tumour cell line (CAC-L153) was transfected with the expression vector and cells with the vector in sense and antisense orientation were selected. Results —Tumour cells with the expression vector in the antisense orientation had a notable reduction in expression of Wnt-1 protein and a considerable reduction in tumour outgrowth compared with controls. Conclusions —The results indicate that the Wnt-1 proto-oncogene may be a possible target for antisense therapy.