Understanding the natural selection of human embryos: Blastocyst quality modulates the inflammatory response during the peri‐implantation period. (4th December 2021)

• Record Type: Journal Article
• Authors: Fernández, Laura; Grasso, Esteban; Soczewski, Elizabeth; Gori, Soledad; Calo, Guillermina; Hauk, Vanesa; Sabbione, Florencia; Gallino, Lucila; Martínez, Gustavo; Irigoyen, Marcela; Bestach, Yesica; Pérez Leirós, Claudia; Ramhorst, Rosanna
• Is Part Of: American journal of reproductive immunology. Volume 87:Number 1(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-12-04
• Subjects: decidualization -- embryo quality -- implantation window; Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.13423
• Languages: English
• ISSNs: 1046-7408

Abstract

Abstract: Problem: Decidualized cells display an active role during embryo implantation sensing blastocyst quality, allowing the implantation of normal developed blastocysts and preventing the invasion of impaired developed ones. Here, we characterized the immune microenvironment generated by decidualized cells in response to soluble factors secreted by blastocysts that shape the receptive milieu. Method of Study: We used an in vitro model of decidualization based on the Human Endometrial Stromal Cells line (HESC) differentiated with medroxiprogesterone and dibutyryl‐cAMP, then treated with human blastocysts‐conditioned media (BCM) classified according to their quality. Results: Decidualized cells treated with BCM from impaired developed blastocysts increased IL‐1β production. Next, we evaluated the ability of decidualized cells to modulate other mediators associated with menstruation as chemokines. Decidualized cells responded to stimulation with BCM from impaired developed blastocysts increasing CXCL12 expression and CXCL8 secretion. The modulation of these markers was associated with the recruitment and activation of neutrophils, while regulatory T cells recruitment was restrained. These changes were not observed in the presence of BCM from normal developed blastocysts. Conclusion: Soluble factors released by impaired developed blastocysts induce an exacerbated inflammatory response associated with neutrophils recruitment and activation, providing new clues to understand the molecular basis of the embryo‐endometrial dialogue.