Photocleavable Fluorescent Membrane Tension Probes: Fast Release with Spatiotemporal Control in Inner Leaflets of Plasma Membrane, Nuclear Envelope, and Secretory Pathway. Issue 1 (23rd November 2021)

• Record Type: Journal Article
• Authors: López‐Andarias, Javier; Eblighatian, Krikor; Pasquer, Quentin T. L.; Assies, Lea; Sakai, Naomi; Hoogendoorn, Sascha; Matile, Stefan
• Is Part Of: Angewandte Chemie international edition. Volume 61:Issue 1(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-11-23
• Subjects: fluorescent probes -- mechanosensitivity -- membrane asymmetry -- membrane tension -- photocleavage; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202113163
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Mechanosensitive flipper probes are attracting interest as fluorescent reporters of membrane order and tension in biological systems. We introduce PhotoFlippers, which contain a photocleavable linker and an ultralong tether between mechanophore and various targeting motifs. Upon irradiation, the original probe is released and labels the most ordered membrane that is accessible by intermembrane transfer. Spatiotemporal control from photocleavable flippers is essential to access open, dynamic or elusive membrane motifs without chemical or physical interference. For instance, fast release with light is shown to place the original small‐molecule probes into the innermost leaflet of the nuclear envelope to image changes in membrane tension, at specific points in time of membrane trafficking along the secretory pathway, or in the inner leaflet of the plasma membrane to explore membrane asymmetry. These results identify PhotoFlippers as useful chemistry tools to enable research in biology. Abstract : Open, dynamic, and elusive membrane motifs are labeled without chemical or physical interference to image their order and tension in living cells: Fast release with light from specific targets liberates the original small‐molecule flipper probe in the innermost leaflet of the nuclear envelope, the inner leaflet of the plasma membrane, or along the secretory pathway to address current questions of biological significance.