Characterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae. (13th August 2021)

• Record Type: Journal Article
• Authors: Lin, Zhenquan; Xu, Kang; Cai, Guang; Liu, Yangqingxue; Li, Yi; Zhang, Zhihao; Nielsen, Jens; Shi, Shuobo; Liu, Zihe
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 48:Number 9/10(2021)
• Publication Date: 2021-08-13
• Subjects: Heterologous expression -- Splicing -- Penicillium species -- Cross-species recognition -- Saccharomyces cerevisiae; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1093/jimb/kuab054
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: Heterologous expression of eukaryotic gene clusters in yeast has been widely used for producing high-value chemicals and bioactive secondary metabolites. However, eukaryotic transcription cis -elements are still undercharacterized, and the cross-species expression mechanism remains poorly understood. Here we used the whole expression unit (including original promoter, terminator, and open reading frame with introns) of orotidine 5′-monophosphate decarboxylases from 14 Penicillium species as a showcase, and analyzed their cross-species expression in Saccharomyces cerevisiae . We found that pyrG promoters from the Penicillium species could drive URA3 expression in yeast, and that inefficient cross-species splicing of Penicillium introns might result in weak cross-species expression. Thus, this study demonstrates cross-species expression from Penicillium to yeast, and sheds light on the opportunities and challenges of cross-species expression of fungi expression units and gene clusters in yeast without refactoring for novel natural product discovery.