Histidine-based hydrogels via singlet-oxygen photooxidation. Issue 48 (23rd November 2021)

• Record Type: Journal Article
• Authors: Liberato, Michelle S.; Cavalcante, Nayara G. S.; Sindu, P. Abinaya; Rodrigues-Jesus, Mônica J.; Zelenovskii, Pavel; Carreira, Ana C. O.; Baptista, Maurício S.; Sogayar, Mari C.; Ferreira, Luís C. S.; Catalani, Luiz H.
• Is Part Of: Soft matter. Volume 17:Issue 48(2021)
• Page Start: 10926
• Page End: 10934
• Publication Date: 2021-11-23
• Subjects: Soft condensed matter -- Periodicals; 530.413
• DOI: 10.1039/d1sm01023a
• Languages: English
• ISSNs: 1744-683X

Abstract

Abstract : The formation of hydrogels by photosensitized oxidation and crosslinking of histidine-derived polymers is demonstrated for the first time. Abstract : The formation of hydrogels by photosensitized oxidation and crosslinking of histidine-derived polymers is demonstrated for the first time. The photooxidation of pendant His mediated by singlet oxygen was used to promote covalent coupling by its dimerization. As a proof-of-concept, two systems were studied: (i) chondroitin sulfate (CS) functionalized with His, and (ii) an elastin-like peptide (ELP) containing His produced by recombinant techniques. Both materials were crosslinked by irradiation at 425 nm in the presence of Zn–porphyrin derivatives yielding His-based hydrogels. The molecular structure and physicochemical properties of ELP–His and other 5 ELPs with photooxidizable amino acids were studied in silica by computer simulation. A correlation between the protein conformation and its elastic properties is discussed. CS–His hydrogels demonstrate larger storage moduli than ELPs with other amino acids. The obtained results show the potential use of photooxidation to create a new type of His-based hydrogels.