Enterococcus innesii sp. nov., isolated from the wax moth Galleria mellonella. Issue 12 (17th December 2021)

• Record Type: Journal Article
• Authors: Gooch, Harriet C. C.; Kiu, Raymond; Rudder, Steven; Baker, David J.; Hall, Lindsay J.; Maxwell, Anthony
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 12(2021)
• Publication Date: 2021-12-17
• Subjects: Enterococcus -- novel species -- Galleria mellonella -- wax moth -- antibiotic resistance -- vancomycin resistant; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005168
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Four bacterial strains were isolated from two different colony sources of the wax moth Galleria mellonella . They were characterized by a polyphasic approach including 16S rRNA gene sequence analysis, core-genome analysis, average nucleotide identity (ANI) analysis, digital DNA–DNA hybridization (dDDH), determination of G+C content, screening of antibiotic resistance genes, and various phenotypic analyses. Initial analysis of 16S rRNA gene sequence identities indicated that strain GAL7 T was potentially very closely related to Enterococcus casseliflavus and Enterococcus gallinarum, having 99.5–99.9 % sequence similarity. However, further analysis of whole genome sequences revealed a genome size of 3.69 Mb, DNA G+C content of 42.35 mol%, and low dDDH and ANI values between the genomes of strain GAL7 T and closest phylogenetic relative E. casseliflavus NBRC 100478 T of 59.0 and 94.5 %, respectively, indicating identification of a putative new Enterococcus species. In addition, all novel strains encoded the atypical vancomycin-resistance gene vanC-4 . Results of phylogenomic, physiological and phenotypic characterization confirmed that strain GAL7 T represented a novel species within the genus Enterococcus, for which the name Enterococcus innesii sp. nov. is proposed. The type strain is GAL7 T (=DSM 112306 T =NCTC 14608 T ).