Antimicrobial resistance profiling and molecular typing of ruminant-borne isolates of Clostridium perfringens from Xinjiang, China. (December 2021)

• Record Type: Journal Article
• Authors: Xiaoting, Wang; Chengcheng, Ning; Chunhui, Ji; Yan, Li; Jing, Li; Qingling, Meng; Jun, Qiao; Lixia, Wang; Kuojun, Cai; Jinsheng, Zhang; Zaichao, Zhang; Weiwei, Yu; Yelong, Peng; Xuepeng, Cai
• Is Part Of: Journal of global antimicrobial resistance. Volume 27(2021)
• Page Start: 41
• Page End: 45
• Publication Date: 2021-12
• Subjects: Clostridium perfringens -- Antimicrobial resistance profiles -- Molecular typing -- Ruminant-borne isolates; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2021.08.003
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: Multidrug resistance (MDR) was frequent among C. perfringens isolates from ruminants. Six genotypes and 33 ST types (ST1-ST33) were identified by ERIC-PCR and MLST typing in isolates, respectively, showing obvious genetic diversity. Type A-I-ST19 was the predominant genotype of C. perfringens isolates from ruminants. The same ST type strains had similar drug resistance, while different ST types exhibited distinct drug resistance. Those isolates drug resistance phenotypes do not exactly correspond to the drug resistance genes that they carry, carrying drug resistance genes but without drug resistance phenotypes may be employed as a "reservoir" for drug resistance genes. Abstract: Objectives: Clostridium perfringens ( C. perfringens ) can cause intestinal diseases in livestock and humans, which seriously threatens the healthy development of animal husbandry and human food safety. Here, the characteristics of antimicrobial resistance and molecular typing of ruminant-borne strains of C. perfringens in Xinjiang, China were explored and profiled. Methods: A total of 307 clinical feces collected from ruminants (cattle and sheep) with diarrheal symptoms were screened for C. perfringens . The recovered isolates were characterized in respect to their antimicrobial resistance pattern and molecular typing. Results: A total of 109 isolates of C. perfringens were isolated from 307 clinical feces of ruminants, most of which displayed the multidrug resistance (MDR) phenotype. Demonstration of the quinolone-resistance gene was the highest among the isolates (70.6%). The multiplex PCR typing based on toxin genes showed that type A and type D strains made up 82.6% (90/109) and 17.4% (19/109), among which, the isolates carrying β2 gene occupied 43.3% (39/90) of type A strains and 31.6% (6/19) of type D strains. These isolates were divided into 6 genotypes (I-VI) by enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) method. A total of 33 ST types (ST1-ST33) were identified by multilocus sequence typing (MLST) method. Conclusion: C. perfringens isolates with multidrug resistance (MDR) were frequent and circulating in ruminants. Among them, type A-Ⅰ-ST19 was the dominant genotype of C. perfringens, displaying obvious genetic diversity. This study provided important epidemiological data for the risk assessment of food safety associated with ruminant-borne C. perfringens in Xinjiang, China.