A "Clickable" Electrodeposited Polymer Films Based on 3‐Ethynylthiophene for the Covalent Immobilization of Proteins. Application to a Label‐free Electrochemical Immunosensor for Escherichia Coli and Staphylococcus Aureus Determination. Issue 12 (22nd September 2021)

• Record Type: Journal Article
• Authors: Svalova, T. S.; Medvedeva, M. V.; Kozitsina, A. N.
• Is Part Of: Electroanalysis. Volume 33:Issue 12(2021)
• Page Start: 2469
• Page End: 2475
• Publication Date: 2021-09-22
• Subjects: Electrochemical polymerization -- 3-ethynylthiophene -- 3, 4-ethylendioxythiophene -- layer-by-layer electrodeposition -- copper catalysed azide-alkyne cycloaddition -- covalent immobilization of proteins -- label-free electrochemical immunosensor; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.202100320
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: An original electrodeposited polymer film, based on 3‐ethynylthiophene, for covalent immobilization of proteins via "click" reaction of copper catalyzed azide‐alkyne cycloaddition was developed. The best characteristics have demonstrated the layer‐by‐layer immobilized 3, 4‐ethylendioxythiophene‐3‐ethynylthiophene composite film. The bovine serum albumin and antibodies have been successfully immobilized on the surface of planar platinum electrode. The approach has been applied to label‐free electrochemical immunosensors for Escherichia coli and Staphylococcus aureus determination. The immunosensors demonstrated LODs 7.2 CFU/mL for Escherichia coli and 15.9 CFU/mL for Staphylococcus aureus, linear range 10 2 –10 6 CFU/mL and could be promising for environmental and food control. Abstract :