Plasma l‐indospicine and 3‐nitropropionic acid in ponies fed creeping indigo: Comparison with results from an episode of presumptive creeping indigo toxicosis. (15th February 2021)

• Record Type: Journal Article
• Authors: Darby, Shannon; Sanchez, L. Chris; Mallicote, Martha F.; House, Amanda M.; Plummer, Caryn E.; Nadruz, Veridiana; Benmoha, Rachel H.; Roberts, Stephen M.; Derendorf, Hartmut; Silva‐Sanchez, Cecilia; Claire, Jami; MacKay, Robert J.
• Is Part Of: Equine veterinary journal. Volume 54:Number 1(2022)
• Page Start: 145
• Page End: 152
• Publication Date: 2021-02-15
• Subjects: horse -- toxicosis -- Indigofera -- creeping indigo -- l‐indospicine -- 3‐nitropropionic acid; Horses -- Diseases -- Periodicals; 636.108905
• DOI: 10.1111/evj.13415
• Languages: English
• ISSNs: 0425-1644

Abstract

Abstract: Background: Creeping indigo ( Indigofera spicata ) toxicosis is an emerging problem among horses in Florida and bordering states. Objectives: To quantify the putative toxins l ‐indospicine (IND) and 3‐nitropropionic acid (NPA) in creeping indigo collected from multiple sites and to measure plasma toxin concentrations in ponies fed creeping indigo and horses with presumptive creeping indigo toxicosis. Study design: Experimental descriptive study with descriptive observational field investigation. Methods: Air‐dried creeping indigo was assayed for IND and NPA content. Five ponies were fed chopped creeping indigo containing 1 mg/kg/day of IND and trace amounts of NPA for 5 days, then observed for 28 days. Blood samples from these ponies and from horses involved in a presumptive creeping indigo toxicosis were assayed for IND and NPA. Results: IND in creeping indigo plants was 0.4‐3.5 mg/g dry matter whereas NPA was <0.01 to 0.03 mg/g. During creeping indigo feeding, clinical and laboratory signs were unchanged except for significant weight loss (median 6%, range 2%‐9%; p = .04) and significant increase from baseline plasma protein concentration (median 16 g/L, range 8‐25 g/L; p < .001). These changes could not definitively be ascribed to creeping indigo ingestion. Plasma IND rose to 3.9 ± 0.52 mg/L on day 6. Pharmacokinetic modelling indicated an elimination half‐life of 25 days and a steady state plasma concentration of 22 mg/L. Plasma IND concentration in sick horses during an incident of creeping indigo toxicosis was approximately twice that of clinically normal pasture mates. Plasma NPA was <0.05 mg/L in all samples. Main limitations: Creeping indigo used in the feeding trial may not be representative of plants involved in creeping indigo toxicosis. There was no control group without creeping indigo in the feeding trial. Conclusions: Indospicine can be detected in blood of horses consuming creeping indigo and the toxin accumulates in tissues and clears slowly. The role of NPA in the neurological signs of this syndrome is unclear.