Development of surface‐enhanced Raman scattering‐based immunoassay platforms using hollow Au nanostars for reliable SARS‐CoV‐2 diagnosis. (14th October 2021)

• Record Type: Journal Article
• Authors: Yu, Qian; Wu, Yixuan; Kang, Taejoon; Choo, Jaebum
• Is Part Of: Bulletin of the Korean Chemical Society. Volume 42:Number 12(2021)
• Page Start: 1699
• Page End: 1705
• Publication Date: 2021-10-14
• Subjects: hollow gold nanostar -- immunoassay -- magnetic bead -- SARS‐CoV‐2 -- surface‐enhanced Raman scattering; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/bkcs.12418
• Languages: English
• ISSNs: 0253-2964

Abstract

Abstract: We developed a novel surface‐enhanced Raman scattering (SERS)‐based SARS‐CoV‐2 assay platform using hollow Au nanostars to realize high‐sensitivity diagnosis of SARS‐CoV‐2. The assay was performed using SARS‐CoV‐2 lysate as the target in a wide dynamic range with virus concentrations ranging from 0 to 10 4 PFU/ml and has a limit of detection (LOD) of 5.1 PFU/ml. This LOD value shows 100 times and 10 times better sensitivity compared to the LODs measured on the same sample using a commercially available rapid kit and enzyme‐linked immunosorbent assay, respectively. Therefore, we believe that this SERS‐based SARS‐CoV‐2 assay platform has high diagnostic accuracy for early or asymptomatic infected patients with low virus concentrations. Furthermore, the probability of a false‐negative diagnosis is likely to be very low. Abstract : A new magnetic bead‐based SERS assay platform was developed to overcome the sensitivity limit of the commercially available SARS‐CoV‐2 immunodiagnostic kits. A novel hollow gold nanostars (HAuNSs), with a high enhancement factor and good stability, was employed for the detection of nucleocapsid protein from SARS‐CoV‐2. The assay could be performed in a wide dynamic range with virus concentrations ranging from 10 −2 to 10 4 PFU/ml and showed an LOD value of 5.1 PFU/ml using this platform.