Quorum sensing signals enhanced caproate production by changing microbial community in chain elongation enrichments. Issue 6 (December 2021)

• Record Type: Journal Article
• Authors: Li, Jing; Liu, He; Wu, Ping; Ding, Peng; Zhang, Jie; Zhang, Chao; Fu, Bo; Cui, Min-hua
• Is Part Of: Journal of environmental chemical engineering. Volume 9:Issue 6(2021)
• Publication Date: 2021-12
• Subjects: Quorum sensing -- N-acyl-homoserine lactones -- Chain elongation -- Caproate -- Microbial community; Chemical engineering -- Environmental aspects -- Periodicals; Environmental engineering -- Periodicals; Chemical engineering -- Environmental aspects; Environmental engineering; Periodicals; 660.0286
• DOI: 10.1016/j.jece.2021.106623
• Languages: English
• ISSNs: 2213-2929

Abstract

Abstract: Quorum sensing (QS) has been widely used to regulate the cell-cell communication. N -acyl-homoserine lactones (AHLs) are typical small-molecule mediating QS among intraspecific communication. In this study, AHLs were investigated to enhance the production of caproate from ethanol and acetate in chain elongation enrichments. N -hexanoyl-L -homoserine lactone (C6-HSL), N -octanoyl-L -homoserine lactone (C8-HSL), and N- (3-oxodecanoyl)-L -homoserine lactone (3OC10- HSL) were added as the signal molecule. Results showed that the highest caproate concentration, 8.46 g/L, was obtained with the addition of C8-HSL (35.14% higher than the control) when ethanol and acetate were substrate. These reinforcement effect of AHLs may be related to enhance content of protein (C8-HSL increased by 466.00 mg/L as relative to the control group) and total biomass (1.99 times of the control group with the addition of C8-HSL). Moreover, the relative abundance of Negativicutes and the concentration of acetyl-CoA were increased, and function prediction of bacterial community suggested that the relative abundance of acetyl-CoA acetyltransferase was improved obviously, which indicated microbial community of chain elongation were more active in presence of AHLs. This study provides an insight to the biotechnology application of chain elongation for caproate production. Graphical Abstract: ga1 Highlights: AHLs promoted caproate production from ethanol and acetate. Higher protein content and total biomass contributed to the reinforcement effect. Negativicutes abundance was increased with the addition of AHLs. AHLs were beneficial to the chain elongation metabolic pathway.