Tetracycline removal in granulation: Influence of extracellular polymers substances, structure, and metabolic function of microbial community. (February 2022)

• Record Type: Journal Article
• Authors: Yan, Lilong; Chen, Wanting; Wang, Caixu; Liu, Shuang; Liu, Cong; Yu, Liangbin; Zheng, Yaoqi; Jiang, Jishuang; Zhang, Yaoli; Xia, Changlei; Lam, Su Shiung
• Is Part Of: Chemosphere. Volume 288:Part 2(2022)
• Publication Date: 2022-02
• Subjects: Aerobic granular sludge -- Tetracycline -- Granulation -- Microbial community -- Metabolic function; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.132510
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Tetracycline is a potentially hazardous residual antibiotic detected in various sewages. High concentration (mg/L) of tetracycline is found in pharmaceutical/hospital wastewater and wastewater derived from livestock and poultry. So far, only antibiotics in μg/L level have been reported in granulation of aerobic sludge during wastewater treatment, but its effects in high concentration are rarely reported. In this study, the influence of tetracycline in high concentration (∼2 mg/L) on the formation of granular sludge, structure, and metabolic function of the microbial community during the granulation of aerobic sludge was investigated to improve the understanding of the aerobic granular sludge formation under high-level of tetracycline. The role of extracellular polymers substances (EPSs) derived from granular sludge in the granulation and tetracycline removal process was also investigated, showing that tetracycline improved the relative hydrophobicity, flocculability and protein/polysaccharide ratio of EPSs, accelerating the granulation of sludge. Succession of microbial communities occurred during the domestication of functional bacteria present in the sludge and was accompanied with regulation of metabolic function. The addition of tetracycline lead to an increase of tetracycline-degrading bacteria or antibiotic resistance genus. Those findings provide new perspectives of the influence of tetracycline on aerobic sludge granulation and the removal mechanism of tetracycline. Graphical abstract: Image 1 Highlights: Tetracycline in EPSs accounted for 40.9% of the tetracycline removal in wastewater. Protein and polysaccharide in loosely-bound EPSs relate to flocculability. Tetracycline influences structure and metabolic function of microbial community. Dokdonella and Thauera arethe potential tetracycline degrading bacteria.