Microcystis aeruginosa's exposure to an antagonism of nanoplastics and MWCNTs: The disorders in cellular and metabolic processes. (February 2022)

• Record Type: Journal Article
• Authors: Zhang, Yafei; Li, Xin; Liang, Jie; Luo, Yuan; Tang, Ning; Ye, Shujing; Zhu, Ziqian; Xing, Wenle; Guo, Jiayin; Zhang, Hui
• Is Part Of: Chemosphere. Volume 288:Part 2(2022)
• Publication Date: 2022-02
• Subjects: Nanoplastics -- Multi-walled carbon nanotubes -- Microcystis aeruginosa -- Transcriptome -- Heterogeneous agglomeration; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.132516
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Nanoplastics and carbon nanotubes (CNTs) is one of the emerging environmental contaminants and a widely used engineering nanomaterial, and their biological toxicity has been frequently studied. However, there has been no research on the combined exposure of these two totally different shape nanoparticles. To explore their potential threat to freshwater ecosystems, Microcystis aeruginosa ( M. aeruginosa ) was exposed to concentration gradients of polystyrene nanoplastics (Nano-PS) and multi-walled carbon nanotubes (MWCNTs). The physiological analysis and whole-transcriptome sequencing were integrated to certify the cytotoxicity. As the physiological results showed, the low concentration (5 mg/L) of these two nanoparticles showed a stimulation on the growth (6.49%–12.2%) and photosynthesis (0–7.6%), and the coexposure was slightly higher than individuals. However, other concentrations showed inhibitory effect, especially at high concentration (50 mg/L), and all physical signs and electron microscope images showed obvious cytotoxicity. Compared with the individuals, the coexposure showed an antagonistic effect induced by the heterogeneous agglomeration which decreased the surface toxicity and the contact with algae of nanomaterials. Transcriptome results showed that coexposure treatment had the fewest differential genes, and the primary effects embodied in the disturbances of cellular and metabolic processes which were superior to the individuals. In the 50 mg/L Nano-PS, the translation process was significantly disordered, and MWCNTs could disrupted the photosynthesis, multiple metabolism processes, membrane transport, and translation. These findings demonstrated the aquatic toxic mechanism from cellular and metabolic processes of Nano-PS and MWCNTs for M. aeruginosa and provided valuable data for environmental risk assessment of them. Graphical abstract: Image 1 Highlights: High concentration nanoplastics exposure significantly disturbed the translation process. MWCNTs disrupted the metabolism, membrane transport, and translation processes. Co-exposure group showed antagonism due to heterogeneous aggregation. Co-exposure group showed slight disorder of cellular and metabolic process.