Co-cultivation of T. asperellum GDFS1009 and B. amyloliquefaciens 1841: Strategy to regulate the production of ligno-cellulolytic enzymes for the lignocellulose biomass degradation. (1st January 2022)

• Record Type: Journal Article
• Authors: Karuppiah, Valliappan; Zhixiang, Lu; Liu, Hongyi; Murugappan, Vallikkannu; Kumaran, Subramanian; Perianaika Anahas, Anthonyraj Matharasi; Chen, Jie
• Is Part Of: Journal of environmental management. Volume 301(2022)
• Publication Date: 2022-01-01
• Subjects: Co-cultivation -- T. asperellum -- B. amyloliquefaciens -- Lignocellulose -- Biomass hydrolysis; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2021.113833
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: The influence of fossil fuels on the environment focused on the development of new technology on biofuels. In this situation, lignocellulolytic hydrolysis enzymes such as Cellobiohydrolase, β-Glucosidase, Endoglucanase, cellulase and xylanase have broad applications in the biofuel production. The Trichoderma have used for the production of cellulase and xylanase to hydrolyze the lignocellulose. Hence, in the present study, co-culture has been employed to induce the production of polysaccharide hydrolyzing enzymes under both induction and repression conditions. The enzyme activity and its gene expression were induced by the co-culture of T. asperellum and B. amyloliquefaciens compared to the monoculture. Further, the co-culture upregulated the transcription regulatory genes and downregulated the repressor genes under both repressor and inducer conditions, respectively. The crude enzyme produced by the co-culture and monocultures using the optimized medium containing molasses, cornmeal and rice bran were further used to hydrolyze the pretreated corn Stover, rice straw, and wheat straw. These results indicate that the co-culture of T. asperellum and B. amyloliquefaciens is a promising and inexpensive method to advance the innovation on the continuous production of cellulase and xylanase under different circumstances for the bioconversion of lignocellulosic biomass into glucose for the bio-fuels. Graphical abstract: Image 1 Highlights: Co-cultivation of T. asperellum and B. amyloliquefaciens upregulated the genes involved in the cellulase production. Co-cultivation induced the expression of genes encoding cellulase and xylanase. The production of cellulolytic enzymes by the co-cultivation was optimized. The crude enzyme from the co-culture effectively hydrolyzed the lignocellulolytic waste biomass.