Study on the differences in sludge toxicity and microbial community structure caused by catechol, resorcinol and hydroquinone with metagenomic analysis. (15th January 2022)

• Record Type: Journal Article
• Authors: Chen, Xiurong; Hu, Xueyang; Lu, Quanling; Yang, Yingying; Linghu, Shanshan; Zhang, Xinyu
• Is Part Of: Journal of environmental management. Volume 302:Part A(2022)
• Publication Date: 2022-01-15
• Subjects: Dihydroxy benzene -- Metagenomics -- Sludge toxicity -- Biodegradation pathway -- Microbial community; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2021.114027
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: The aerobic biodegradation rate, organic toxicity and microbial community structure of activated sludge acclimated by catechol, resorcinol and hydroquinone were investigated, to study the relationship between microbial structure and sludge organic toxicity caused by phenolic compounds. At the stable operation stage, the degradation rates of the dihydroxy benzenes in a single sequencing batch reactor (SBR) cycle were followed the order: resorcinol (89.71%) > hydroquinone (85.64%) > catechol (59.62%). Sludge toxicity bioassay indicated that the toxicity of sludge was catechol (45.63%) > hydroquinone (40.28%) > resorcinol (38.15%). The accumulation of secondary metabolites such as 5–10 kDa tryptophan and tyrosine protein substances caused the differential sludge toxicity. Microbial metagenomic analysis showed that the toxicity of sludge was significantly related to the microbial community structure. Thauera, Azoarcus, Pseudomonas and other Proteobacteria formed in the sludge during acclimation. Catechol group had the least dominant bacteria and loop ring opening enzyme genes ( catA, dmpB, dxnF, hapD ) numbers. Therefore, the degradation of catechol was the most difficult than resorcinol and hydroquinone, resulting the highest sludge toxicity. Graphical abstract: Image 1 Highlights: Loop ring opening enzyme genes affected the degradation of dihydroxy benzenes. The degradation rates followed the order resorcinol > hydroquinone > catechol. Sludge organic toxicity contrasted degradation rates of influent dihydroxy benzenes. 5–10 kDa tryptophan and tyrosine protein caused differential sludge toxicity.