Real-time tracking of delayed-onset cellular apoptosis induced by intracellular magnetic hyperthermia. (January 2016)

• Record Type: Journal Article
• Authors: Blanco-Andujar, Cristina; Ortega, Daniel; Southern, Paul; Nesbitt, Stephen A; Thanh, Nguyễn Thị Kim; Pankhurst, Quentin A
• Is Part Of: Nanomedicine. Volume 11:Number 2(2016)
• Page Start: 121
• Page End: 136
• Publication Date: 2016-01
• Subjects: apoptosis -- cell death pathways -- human melanoma cells -- magnetic hyperthermia -- magnetic nanoparticles; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.15.185
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To assess cell death pathways in response to magnetic hyperthermia.Materials & methods: Human melanoma cells were loaded with citric acid-coated iron-oxide nanoparticles, and subjected to a time-varying magnetic field. Pathways were monitored in vitro in suspensions and in situ in monolayers using fluorophores to report on early-stage apoptosis and late-stage apoptosis and/or necrosis.Results: Delayed-onset effects were observed, with a rate and extent proportional to the thermal-load-per-cell. At moderate loads, membranal internal-to-external lipid exchange preceded rupture and death by a few hours (the timeline varying cell-to-cell), without any measurable change in the local environment temperature.Conclusion: Our observations support the proposition that intracellular heating may be a viable, controllable and nonaggressive in vivo treatment for human pathological conditions.